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D5688

Sigma-Aldrich

Dilution Solution

Extract-N-Amp Plant Dilution Solution

Synonym(s):

Direct PCR, Direct PCR dilution solution for plants, Extract‑N‑Amp Dilution Solution

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.55

Quality Level

200

form

liquid

storage temp.

−20°C

General description

Dilution Solution is intended for use with Sigma′s Extract-N-Amp Plant PCR Kit. Once the DNA is extracted from the leaf tissue, the Dilution Solution is added to the extract to neutralize inhibitory substances. The extract is then ready for PCR.

Application

Dilution Solution (Extract-N-Amp™ Plant Dilution Solution) has been used in direct PCR amplification and to prepare template DNA.

Legal Information

Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC

Related product

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XNAP

REDExtract-N-Amp™ Plant PCR Kit, sufficient for 100 extractions, sufficient for 100 amplifications

XNAPE

REDExtract-N-Amp™ Plant PCR Kit, sufficient for 100 extractions, sufficient for 500 amplifications

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Extract-N-Amp™ Plant PCR Kit, sufficient for 100 extractions, sufficient for 100 amplifications

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XNAPS

REDExtract-N-Amp™ Plant PCR Kit, sufficient for 10 extractions, sufficient for 10 amplifications

R4775

REDExtract-N-Amp™ PCR ReadyMix™, Ready-to-use 2X PCR Master Mix with Loading Dye

E3004

Extract-N-Amp™ PCR ReadyMix™, Amplifications to support Extract-N-Amp Plant and Extract-N-Amp Tissue

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Extract-N-Amp™ PCR Diluent, colorless, storage temp.:2-8°C

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SYBR® Green Extract-N-Amp™ PCR ReadyMix™, Amplifications to support Extract-N-Amp Plant and Extract-N-Amp Tissue

E0781

Extract-N-Amp™ PCR ReadyMix™ with qPCR Reference dye, 125 mL sufficient for 10000 amplifications, hotstart, dNTPs included

pictograms

Exclamation markEnvironment
GHS07,GHS09

signalword

Warning

Hazard Classifications

Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Jose L Ramirez et al.
Journal of insect science (Online), 8, 1-7 (2008-04-01)
Homalodisca vitripennis (Germar) (Hemiptera: Cicadellidae), the glassy-winged sharpshooter, is one of the most important vectors of the bacterium, Xylella fastidiosa subsp. piercei (Xanthomonadales: Xanthomonadaceae) that causes Pierce's Disease in grapevines in California. In the present study we report a new
Molecular Diagnosis of Urinary Tract Infections by Semi-Quantitative Detection of Uropathogens in a Routine Clinical Hospital Setting.
van der Zee A
PLoS ONE, 11(3), e0150755-e0150755 (2016)
Anneke van der Zee et al.
BMC infectious diseases, 14, 27-27 (2014-01-16)
Resistance to carbapenem antibiotics is emerging worldwide among Enterobacteriaceae. To prevent hospital transmission due to unnoticed carriage of carbapenemase producing micro-organisms in newly admitted patients, or follow-up of patients in an outbreak setting, a molecular screening method was developed for
Anneke van der Zee et al.
BMC research notes, 4, 150-150 (2011-05-28)
To facilitate automation, a novel DNA extraction method for MRSA was adopted. The MRSA specific chromosome-SCCmec PCR was adapted, additional primers were added, and the performance was validated. From various laboratories in The Netherlands we received a total of 86
Daniela Hegebarth et al.
Plant, cell & environment, 40(9), 1761-1776 (2017-05-10)
The aliphatic waxes sealing plant surfaces against environmental stress are generated by fatty acid elongase complexes, each containing a β-ketoacyl-CoA synthase (KCS) enzyme that catalyses a crucial condensation forming a new C─C bond to extend the carbon backbone. The relatively
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Protocols

REDExtract-N-Amp™ Plant PCR Kit Protocol

The REDExtract-N-Amp Plant PCR kit includes an inert loading dye in the ReadyMix for easy gel loading after amplification. This protocol provides a rapid protocol to extract plant genomic DNA for amplification using the optimized ReadyMix.

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