CS0004

Nuclease micrococcal from Staphylococcus aureus

With reaction buffer and BSA solution

• Synonym(s): Nuclease micrococcal from Staphylococcus aureus, Endonuclease micrococcal, MNase, Micrococcal endonuclease
• CAS Number: 9013-53-0
• Enzyme Commission number: 3.1.31.1 (BRENDA, IUBMB)
• MDL number: MFCD00131731
• UNSPSC Code: 12352202
• NACRES: NA.77

Properties

• biological source: Staphylococcus aureus
• form: liquid
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

Nuclease Micrococcal (MNase) is the extracellular nuclease of Staphylococcus aureus. The enzyme is an endo-exonuclease .This Endo-exonuclease preferentially digests single-stranded nucleic acids. Thus, nuclease micrococcal activity results in the production of mononucleotides and oligonucleotides with terminal 3′-phosphates. Nuclease Micrococcal is also active against double-stranded DNA and RNA and all sequences will be ultimately cleaved.
Micrococcal Nuclease can be used for the digestion of chromatin to identify the location of nucleosomes

Application

• Chromatin Immuno-Percipitation (ChIP) analysis
• Nucleosome preparation
• Removal of nucleic acids present in protein preparation
• Reduction of viscosity of cell lysates during non-mechanical cell lysis preparation
• RNA sequencing and transcriptomic profiling

Biochem/physiol Actions

Hydrolyzes 5′-phosphodiester bonds of DNA.

Other Notes

Note: One μmolar unit = approx. 85 A₂₆₀ units, where an A₂₆₀ unit is equivalent to a ΔA₂₆₀ of 1.0 in 30 min at pH 8.8 at 37 °C in a 3.55 mL reaction volume. Light path = 1 cm.

Safety Information

• Storage Class: 10 - Combustible liquids