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CCF100

Sigma-Aldrich

CM Sepharose™

Fast Flow

Synonym(s):

Carboxymethyl Sepharose™

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About This Item

CAS Number:
68894-07-5
MDL number:
MFCD00146478
UNSPSC Code:
47101511
NACRES:
NA.56

form

suspension

technique(s)

affinity chromatography: suitable

matrix

6% cross-linked agarose

particle size

45-165 μm (wet bead)

pore size

4,000,000 exclusion limit (av. mol. wt.)

pH

4—13

capacity

90-130 μeq/mL(gel basis)

storage temp.

2-8°C

General description

Preswollen in 20% ethanol
CCF100-500ml′s updated product number is GE17-0719-01
25 ml is GE17-0719-10

Application

CM Sepharose™ is used in affinity chromatography, protein chromatography and ion exchange chromatography. CM Sepharose™ hase been used to study diet supplements and to characterize biochemical and enzymatic properties of Viperidae venom.

Legal Information

Sepharose is a trademark of Cytiva

Replaced by

Product No.
Description
Pricing

pictograms

Flame
GHS02

signalword

Warning

hcodes

H226

Hazard Classifications

Flam. Liq. 3

Storage Class

3 - Flammable liquids

wgk_germany

WGK 1

flash_point_f

100.4 - 109.4 °F

flash_point_c

38 - 43 °C

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Meifang Xiao et al.
Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences, 36(11), 1065-1070 (2011-12-16)
To purify and identify HMGB1 secreted by liver cells HepG2 and immune cells U937. We cultured the liver cell lines HepG2 and immune cell lines U937, and stimulated them with HMGB1 (400 ng/mL) for 20 h. Then the supernatant was
Balsamin, a novel ribosome-inactivating protein from the seeds of Balsam apple Momordica balsamina.
Kaur, I., et al.
Amino Acids (2011)
Fei Wang et al.
Methods (San Diego, Calif.), 53(3), 208-213 (2010-12-24)
The infectivity associated with prion disease sets it apart from a large group of late-onset neurodegenerative disorders that shares the characteristics of protein aggregation and neurodegeneration. The unconventional infectious agent, PrP(Sc), is an aberrantly folded form of the normal prion
Biochemical and enzymatic characterization of BpMP-I, a fibrinogenolytic metalloproteinase isolated from Bothropoides pauloensis snake venom.
Naves de Souza, D.L., et al.
Comp. Biochem. Physiol., B: Comp. Biochem., 161, 102-109 (2012)
Tuo Wang et al.
Plant physiology, 172(4), 2107-2119 (2016-10-13)
The wall-loosening actions of β-expansins are known primarily from studies of EXPB1 extracted from maize (Zea mays) pollen. EXPB1 selectively loosens cell walls (CWs) of grasses, but its specific binding target is unknown. We characterized EXPB1 binding to sequentially extracted
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