ND7/23 Cell Line

92090903, Mouse x Rat Hybridoma nerve, Neuronal

• Synonym(s): ND7/23 Cells
• UNSPSC Code: 41106514

Properties

• Product Name: ND7/23 Cell Line, 92090903, Mouse neuroblastoma X Rat neuron hybrid
• biological source: Mouse x Rat Hybridoma nerve
• growth mode: Adherent
• karyotype: Not specified
• morphology: Neuronal
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Mouse neuroblastoma x Rat neurone hybrid

Cell Line Description

Mouse neuroblastoma (N18 tg 2) x rat dorsal root ganglion neurone hybrid cell line produced by PEG mediated cell fusion.

Application

Mouse neuroblastoma (N18 tg 2) and rat dorsal root glanglia produced by PEG-mediated fusion.

Recommended culture medium: DMEM + 2 mM L-Glutamine + 10% FBS

Subculture: Split at 70-80% confluency, approx. 1:3 to 1:6, seed at 1-3x10⁴ viable cells/cm² at 37°C and 5% CO₂. Dissociate cells by aspiration or gentle tapping. Cells are lightly adherent, no trypsinization is necessary.

Culture Medium

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-3x10,000 cells/cm² by either aspirating or gently tapping the cells loose; 5% CO₂; 37°C. Cells are lightly attached therefore use of trypsin is unnecessary.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.