HCT 116 Cell Line human

91091005, human colon, Epithelial-like

• Synonym(s): HCT116 Cells
• UNSPSC Code: 41106514

Properties

• product name: HCT 116 Cell Line human, 91091005, colon carcinoma
• biological source: human colon
• growth mode: Adherent
• karyotype: Modal no. 45
• morphology: Epithelial-like
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Human colon carcinoma

Cell Line Description

One of 3 strains of malignant cells isolated from a male with colonic carcinoma. Cells are tumourigenic in nude mice and form colonies on agarose. Growth and plating efficiency are enhanced by using a feeder layer of murine fibroblasts.

Application

HCT 116 Cell Line has been used to:

• study the importance of cyclin D1 for the activity of lithocholic acid hydroxyamide (LCAHA)
• study the effects of different amounts of transferrin within the polyethyleneimine (PEI)-small interfering RNA (siRNA) complexes on the uptake of fluorescently-labelled siRNA
• determine the effect of PTS (polyamine transport system) in the selective uptake of Quilamine HQ1-44, an iron chelator

Tumourigenicity studies

DNA Profile

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 7,10
D13S317: 10,12
D16S539: 11,13
D5S818: 10,11
D7S820: 11,12
THO1: 8,9
TPOX: 8
vWA: 17,22

Culture Medium

McCoy′s 5a + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

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