B3111
ANTI-FLAG® M2 antibody, Mouse monoclonal
Clone M2, purified from hybridoma cell culture in bioreactor
Synonym(s):
Anti-ddddk, Anti-dykddddk, M2 clone ANTI-FLAG
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About This Item
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biological source
mouse
antibody form
purified immunoglobulin (purified IgG1 subclass)
clone
M2, monoclonal
shelf life
4 yr
purified by
using Protein A
storage temp.
−20°C
General description
Monoclonal ANTI-FLAG M2 is a purified immunoglobulin, IgG1, monoclonal antibody, purified from culture supernatant of hybridoma cells, that binds to FLAG® fusion proteins. Unlike ANTI-FLAG M1 antibody, the M2 antibody will recognize the FLAG sequence at the N-terminus, Met-N-terminus, C-terminus, or at an internal site of FLAG fusion proteins. Monoclonal ANTI-FLAG M2 is useful for identification and capture of FLAG fusion proteins by common immunological procedures such as Western blots and immunoprecipitation. It is also useful for affinity purification of FLAG fusion proteins when bound to a solid support.
form: solution pH 7.4, containing 15 mM sodium azide
concentration: 3.0-5.0 mg/mL
form: solution pH 7.4, containing 15 mM sodium azide
concentration: 3.0-5.0 mg/mL
Application
IB, IF, IP, FACS, ELISA
Antibody is recommended for use in several applications such as immunoblotting, immunoprecipitation, immunofluorescence, flow cytometry, and ELISA.
Learn more product details in our FLAG® application portal.
Antibody is recommended for use in several applications such as immunoblotting, immunoprecipitation, immunofluorescence, flow cytometry, and ELISA.
Learn more product details in our FLAG® application portal.
Packaging
polypropylene screw cap vial
Preparation Note
Dilute the antibody solution from 0.5-10 ug/mL in specified buffer
Legal Information
ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class
12 - Non Combustible Liquids
wgk_germany
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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