A2981

Anti-ATG4B antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• Synonym(s): Anti-APG4B, Anti-AUT-like 1 cysteine endopeptidase, Anti-AUTL1, Anti-Autophagin-1, Anti-Autophagy-related cysteine endopeptidase 1, Anti-Autophagy-related protein 4 homolog B, Anti-Cysteine protease ATG4B, Anti-KIAA0943, Anti-hAPG4B, ATG4
• MDL number: MFCD09753846
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 200
• conjugate: unconjugated
• antibody form: IgG fraction of antiserum
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~44 kDa
• species reactivity: mouse, rat, human
• technique(s): immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50-1:100 using biotin/ExtaAvidin^®-Peroxidase staining of heat-retrieved human cerebellum sections; western blot: 1:125-1:500 using whole extract of 3T3 cells; western blot: 1:500-1:1,000 using whole extract of rat brain
• UniProt accession no.: Q9Y4P1
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... ATG4B(23192) ; mouse ... Atg4b(66615) ; rat ... Atg4b(316640)

Description

General description

ATG4B (autophagy-related 4B cysteine peptidase), a mammalian orthologue of yeast Atg4, is a cysteine protease. It is widely expressed in human and rat tissues, being most abundant in the brain. This gene is mapped to human chromosome 2q37.

ATG4B is a member of the C-54 family of cysteine proteases that is involved in cell degradation through autophagy.

Immunogen

synthetic peptide corresponding to amino acids 6-22 of human ATG4B, conjugated to KLH via a C-terminal cysteine residue. The corresponding sequence is identical in mouse.

Application

Anti-ATG4B antibody produced in rabbit has been used in

• western blotting
• immunohistochemistry

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)
Western Blotting (1 paper)

Biochem/physiol Actions

ATG4B (autophagy-related 4B cysteine peptidase) participates in the modulation of autophagy in mammalian cells. It is also involved in light chain 3 (LC3) lipidation. ATG4B is responsible for the processing of the mammalian Atg8 homologues: Golgi-associated ATPase enhancer of 16 kDa (GATE16), gamma-aminobutyric-acid-type-A-receptor-associated protein (GABARAP), and LC3. It removes the carboxyl-terminal region of proLC3 immediately after its synthesis, generating the soluble LC3-I and exposing a carboxyl-terminal Gly.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Legal Information

ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)