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90819

Sigma-Aldrich

Bis(2,2′-bipyridine)-(5-aminophenanthroline)ruthenium bis(hexafluorophosphate)

BioReagent, suitable for fluorescence

Synonym(s):

Ru(bpy)2(phen-5-NH2)(PF6)2

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About This Item

Empirical Formula (Hill Notation):
C32H25N7Ru · 2 PF6
CAS Number:
84537-86-0
Molecular Weight:
898.59
MDL number:
MFCD20921517
UNSPSC Code:
12171500
PubChem Substance ID:
57653411
NACRES:
NA.21

product line

BioReagent

form

powder

reaction suitability

core: ruthenium
reaction type: Photocatalysis
reagent type: catalyst

fluorescence

λex 458 nm; λem 612 nm in 0.1 M Tris pH 7.0

suitability

suitable for fluorescence

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

−20°C

SMILES string

[Ru++].F[P-](F)(F)(F)(F)F.F[P-](F)(F)(F)(F)F.c1ccc(nc1)-c2ccccn2.c3ccc(nc3)-c4ccccn4.Nc5cc6cccnc6c7ncccc57

InChI

1S/C12H9N3.2C10H8N2.2F6P.Ru/c13-10-7-8-3-1-5-14-11(8)12-9(10)4-2-6-15-12;2*1-3-7-11-9(5-1)10-6-2-4-8-12-10;2*1-7(2,3,4,5)6;/h1-7H,13H2;2*1-8H;;;/q;;;2*-1;+2

InChI key

QQMMCXKCYXQNNA-UHFFFAOYSA-N

Application

Used for photoinduced intramolecular electron transfer in peptide-bridged molecules.

Features and Benefits

Sigma-Aldrich offers a series of reactive dyes based on ruthenium complexes for use in fluorescence polarization assays and fluorescence lifetime imaging applications. They have been designed specifically to have long fluorescence lifetimes. These dyes were first synthesized in the labs of Dr. Joseph Lakowicz at the Center for Fluorescence Microscopy. These laser-excitable metal ligand complexes have fluorescence lifetimes near 500 nanoseconds.
These dyes can be used to label amines on biomolecules under mild conditions. Advantages of these dyes as protein labels include high photostability, good water solubility, a lack of dye-dye interactions and large Stokes′ shifts.
In addition, the fluorescence signal of long-lived fluorophores can be gated to eliminate the emission from short-lifetime fluorophores and autofluorescence from cells and biomolecules to further improve sensitivity.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Other Notes

Photoinduced intramolecular electron transfer in peptide-bridged molecules

related product

Product No.
Description
Pricing

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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E Terpetschnig et al.
Analytical biochemistry, 251(2), 241-245 (1997-09-23)
We report the synthesis and spectral properties of a long-lifetime luminescent Ru complex containing a sulfhydryl-reactive maleimide group, [Ru (2,2'-bipyridine)2(1, 10-phenanthroline-5-maleimide)](PF6)2. [Ru(bpy)2(phen-mi)]2+ was covalently linked to human serum albumin, immunoglobulin G, and beta-galactosidase. The lifetimes for probe bound to proteins
Tao Wang et al.
Analytica chimica acta, 1062, 124-130 (2019-04-06)
Vibrio parahaemolyticus (VP), as a kind of harmful bacterium to human beings, its rapid and accurate detection is particularly important. Herein, we developed a simple and efficient dual-modal Faraday cage-type immunosensor for ultrasensitive detection of VP based on electrochemiluminescence (ECL)
K.S. Schanze et al.
Journal of the American Chemical Society, 110, 1180-1180 (1988)

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