E9379

Stains-All

~95%

• Synonym(s): 1-Ethyl-2-[3-(1-ethylnaphtho[1,2-d]thiazolin-2-ylidene)-2-methylpropenyl]naphtho[1,2-d]thiazolium bromide, 3,3′-Diethyl-9-methyl-4,5,4′,5′-dibenzothiacarbocyanine
• Empirical Formula (Hill Notation): C₃₀H₂₇BrN₂S₂
• CAS Number: 7423-31-6
• Molecular Weight: 559.58
• Beilstein/REAXYS Number: 3865213
• EC Number: 231-047-7
• MDL number: MFCD00078788
• UNSPSC Code: 12171500
• PubChem Substance ID: 24894694
• NACRES: NA.47

Properties

• assay: ~95%
• Quality Level: 200
• form: powder
• solubility: chloroform: 40 mg (PLUS 4.0 ml)
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: 2-8°C
• SMILES string: [Br-].CCN1C(/Sc2ccc3ccccc3c12)=C\C(C)=C\c4sc5ccc6ccccc6c5[n+]4CC
• InChI: 1S/C30H27N2S2.BrH/c1-4-31-27(33-25-16-14-21-10-6-8-12-23(21)29(25)31)18-20(3)19-28-32(5-2)30-24-13-9-7-11-22(24)15-17-26(30)34-28;/h6-19H,4-5H2,1-3H3;1H/q+1;/p-1
• InChI key: MPBRYMWMMKKRGC-UHFFFAOYSA-M

Description

Application

Stains-All has been used for the staining of gel made for size determination of hyaluronan. It has also been used for the staining of DNA oligonucleotides separated by nondenaturing polyacrylamide gel electrophoresis.

Biochem/physiol Actions

Stains-All is a cationic carbocyanine dye and is a multipurpose dye. It stains sialoglycoproteins blue, Ca²⁺ binding proteins deep blue to violet, proteins red and lipids yellow-orange. It also stains RNA (bluish purple) and DNA (blue).

As little as 3 ng (123 BP fragment) of pBR322/Hae III digest DNA and 90 ng tRNA can be detected on a polyacrylamide gel. PAGE gels are stained in the dark and then destaining by removing the gel from the staining solution and exposing it to the light from a light box until sufficient destaining has occurred. A staining solution is typically made by dissolving the dye in formamide and buffer.

Caution

Upon continued exposure to light the nucleic acid-dye complex will fade and disappear.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)