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D2535

Sigma-Aldrich

Direct Blue 15

suitable for Histopaque® system, Powder

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About This Item

Linear Formula:
C34H24N6O16S4Na4
CAS Number:
2429-74-5
Molecular Weight:
992.80
Colour Index Number:
24400
EC Number:
219-385-3
MDL number:
MFCD00036441
UNSPSC Code:
12171500
PubChem Substance ID:
24893697
NACRES:
NA.47

product name

Direct Blue 15, suitable for Histopaque® system, suitable for viability studies of collagenase-treated rat liver cells

description

suitable for Histopaque® system

Quality Level

200

form

powder

composition

Dye content, ~44%

color

dark blue

solubility

water: 10 mg/mL, clear, blue

suitability

suitable for viability studies of collagenase-treated rat liver cells

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].[Na+].[Na+].[Na+].COc1cc(ccc1N=Nc2c(O)c3c(N)cc(cc3cc2S([O-])(=O)=O)S([O-])(=O)=O)-c4ccc(N=Nc5c(O)c6c(N)cc(cc6cc5S([O-])(=O)=O)S([O-])(=O)=O)c(OC)c4

InChI

1S/C34H28N6O16S4.4Na/c1-55-25-9-15(3-5-23(25)37-39-31-27(59(49,50)51)11-17-7-19(57(43,44)45)13-21(35)29(17)33(31)41)16-4-6-24(26(10-16)56-2)38-40-32-28(60(52,53)54)12-18-8-20(58(46,47)48)14-22(36)30(18)34(32)42;;;;/h3-14,41-42H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;/q;4*+1/p-4

InChI key

OLSOUGWNONTDCK-UHFFFAOYSA-J

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Application

May be used as a substitute for trypan blue for some biological and staining applications.

Legal Information

Histopaque is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H350

Hazard Classifications

Carc. 1B

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
015F00601

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A Kai et al.
International journal of biological macromolecules, 20(3), 221-231 (1997-06-01)
The difference of influence of a certain kind of direct dye on the structure of nascent microbial cellulose was examined, with Direct Red 28 have a biphenylenebis(azo) skeletal structure; Direct Blue 1 having two hydroxyl, two methoxy and two sulfonate
A Shan et al.
Journal of chromatographic science, 26(9), 439-442 (1988-09-01)
Two structurally similar azo dyes, Direct Blue No. 6 and Direct Blue No. 15, are analyzed by separate reversed-phase high-performance liquid chromatographic (HPLC) systems utilizing a dual-wavelength UV/visible detector at 254 and 546 nm. Each dye contains more than 35
Metabolism of benzidine and benzidine-congener based dyes by human, monkey and rat intestinal bacteria.
C E Cerniglia et al.
Biochemical and biophysical research communications, 107(4), 1224-1229 (1982-08-31)
Koel Kumar et al.
Bioresource technology, 98(16), 3168-3171 (2007-02-27)
Studies were carried out on decolorisation and biotransformation of the dye Direct Blue-15 into 3,3'-dimethoxybenzidine (O'-dianisidine) and a sulphonated derivative by a five-member bacterial consortium. Chromatographic studies revealed further complete biodegradation of 3,3'-dimethoxybenzidine coupled with release of ammonia, but the
Chih-Huang Weng et al.
Ultrasonics sonochemistry, 20(3), 970-977 (2012-11-29)
Decolourization of direct azo dye, direct blue 15 (DB15), by an advanced Fenton process coupled with ultrasonic irradiation (Fenton/US) was investigated. Zero-valent iron (ZVI) aggregates were used as the catalyst. A positive synergistic effect occurred when Fenton's reagent was combined
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