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ATG004

Sigma-Aldrich

A375 NY−ESO−1 Antigen Panel Cell Lines

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About This Item

UNSPSC Code:
41106514
NACRES:
NA.41

biological source

human skin (malignant melanoma)

growth mode

adherent

morphology

epithelial

shipped in

dry ice

storage temp.

−196°C

General description

This product is a panel of three (3) genetically modified cell lines involving the NY-ESO-1 gene. The panel consists of a NY-ESO-1 knockout cell line (NY-ESO-1-/-) as well as a NY-ESO-1low and a NY-ESO-1high expression cell line. The NY-ESO-1-/- cell line was generated using CompoZr® Zinc Finger Nuclease technology, and the two NY-ESO-1 expression cell lines were generated using MISSION® lentivirus. The NY-ESO-1low and NY-ESO-1high cell lines express NY-ESO-1 at low and high levels relative to the NY-ESO-1-/- cell line (NY-ESO-1high > NY-ESO-1low > NY-ESO-1-/-) as measured via fluorescence-activated cell sorting (FACS). As a comparison, wild type A375 cells demonstrate NY-ESO-1medium expression as measured via FACS.

The STR profile of these cell lines match that of its parental cell line - ECACC catalog number 88113005.

Cell Line Description

Derived from a 54 year old female with malignant melanoma. The cell line produces rapidly growing amelanotic melanomas in anti-thymocyte serum treated NIH Swiss mice.

Features and Benefits

The A375 NY-ESO-1 antigen panel cell lines feature NY-ESO-1 expressed at varying levels (KO, low, and high). These cell lines enable researchers to screen and evaluate the efficacy and potency of NY-ESO-1-targeted immunotherapies in a biologically-relevant cellular research model.

Components

ATG004A-1VL: A375 NY-ESO-1 Knockout
ATG004B-1VL: A375 NY-ESO-1 Low
ATG004C-1VL: A375 NY-ESO-1 High

Quality

Tested for Mycoplasma, bacterial and fungal content, post-freeze viability, and short terminal repeat (STR) analysis for cell line identification.

Legal Information

MISSION Use Licenses Agreement

ZFN Use Licenses Agreement
CompoZr is a registered trademark of Merck KGaA, Darmstadt, Germany
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

10 - Combustible liquids

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