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ATG002

Sigma-Aldrich

A549 EGFR Antigen Panel Cell Lines

Synonym(s):

A549 Cell Panel, A549 EGFR Cell Lines, EGFR Antigen Panel

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About This Item

UNSPSC Code:
41106514
NACRES:
NA.81

biological source

human lung (carcinoma)

growth mode

adherent

morphology

epithelial

shipped in

dry ice

storage temp.

−196°C

General description

This product is a panel of three (3) genetically modified cell lines involving the EGFR gene. The panel consists of a EGFR knockout cell line (EGFR-/-) as well as a EGFRlow and a EGFRhigh expression cell line. The EGFR-/- cell line was generated using CompoZr Zinc Finger Nuclease technology, and the two EGFR expression cell lines were generated using MISSION® lentivirus. The EGFRlow and EGFRhigh cell lines express EGFR at low and high levels relative to the EGFR-/- cell line (EGFRhigh > EGFRlow > EGFR-/-) as measured via fluorescence-activated cell sorting (FACS). As a comparison, wild type A549 cells demonstrate EGFRmedium expression as measured via FACS.

The STR profile of these cell lines match that of its parental cell line - ECACC catalog number 86012804.

Cell Line Description

Derived from a 58 year old Caucasian male. The cells can synthesise lecithin utilising the cytidine diphosphocholine pathway. Occasional cells may also contain inclusion bodies although they are not known to carry any human pathogen.

Features and Benefits

The A549 EGFR antigen panel cell lines feature EGFR expressed at varying levels (KO, low, and high). These cell lines enable researchers to screen and evaluate the efficacy and potency of EGFR-targeted immunotherapies in a biologically-relevant cellular research model.

Components

ATG002A-1VL: A549 EGFR Knockout
ATG002B-1VL: A549 EGFR Low
ATG002C-1VL: A549 EGFR High

Quality

Tested for Mycoplasma, bacterial and fungal content, post-freeze viability, and short terminal repeat (STR) analysis for cell line identification.

Legal Information

Combigel is a trademark of Sigma-Aldrich Co. LLC
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

10 - Combustible liquids


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Whether you need screening tools for CAR/TCR therapies and mAB development, target cells for efficacy testing, or potency assays for clinical development, we provide functional biologically-relevant solutions to help you save time, accelerate your immuno-oncology research, and simplify your workflow.

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