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PGI-RO

Roche

Phosphoglucose Isomerase (PGI)

from yeast

Synonym(s):

glucose-6-phosphate isomerase

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About This Item

Enzyme Commission number:
UNSPSC Code:
12352204

biological source

yeast

Quality Level

form

suspension

specific activity

~350 units/mg protein (at 25 °C with fructose-6-P as the substrate.)

packaging

pkg of 1 mL (10127396001 [2 mg])
pkg of 1 mL (10128139001 [10 mg])

manufacturer/tradename

Roche

optimum pH

7.0-7.6

storage temp.

2-8°C

Related Categories

General description

Phosphoglucose Isomerase is an enzyme which possesses diagnostic potential when fractionated by cellulose acetate electrophoresis. This cytosolic enzyme plays an important role during glycolysis and glucogenesis. It acts as an tumor-secreted cytokine and autocrine motility factor. It is used as neurotrophic factor, maturation factor and as serine proteinase inhibitor.

Specificity

Phosphoglucose isomerase (PGI) is specific for G6P (Km = 0.7 mM) and fructose-6-phosphate (F6P).

Application

Isomerization of ketoses to aldoses.

Quality

Contaminants: <0.01% F6P-K, GR, 6-PGDH and PGluM, each, <0.2% β-fructosidase

Physical form

Suspension in 3.2 M ammonium sulfate solution, pH approximately 6

Preparation Note

Storage conditions (working solution): A solution of PGI in 20 mM Tris, pH 8.1, containing 0.2 mg BSA/ml, is stable approx. 1 week at 2 to 8 °C.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

No data available

flash_point_c

No data available


Certificates of Analysis (COA)

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Aamir Ahmad et al.
Cancer research, 71(9), 3400-3409 (2011-03-11)
Phosphoglucose isomerase/autocrine motility factor (PGI/AMF) plays an important role in glycolysis and gluconeogenesis and is associated with invasion and metastasis of cancer cells. We have previously shown its role in the induction of epithelial-mesenchymal transition (EMT) in breast cancer cells

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