10799050001
Roche
BM-Cyclin
lyophilized, active toward mycoplasma
Synonym(s):
antibiotic, minocycline, tiamulin
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About This Item
UNSPSC Code:
12352207
Recommended Products
form
lyophilized
Quality Level
packaging
pkg of 37.5 mg (for 2 x 2.5 l medium)
manufacturer/tradename
Roche
antibiotic activity spectrum
mycoplasma
mode of action
protein synthesis | interferes
storage temp.
2-8°C
General description
BM-Cyclin is a combination of two antibiotics, namely tiamulin and minocycline, both of which prevent protein synthesis.
Specificity
Sensitive Organisms: Mycoplasmas and bacteria
Note: These mycoplasma strains account for more than 85% of the contaminations in animal cell cultures.
- BM Cyclin was found to effectively eliminate Acholesplasma laidlawii,
- Mycoplasma arginini,
- Mycoplasma hyorhinis, and
- Mycoplasma orale
Note: These mycoplasma strains account for more than 85% of the contaminations in animal cell cultures.
Application
BM-Cyclin is used for the elimination of mycoplasma from infected cell cultures without obvious marked cytotoxic side effects.
Packaging
Set of two different antibiotics
Physical form
Vial 1 (Tiamulin-hydrogenfumarat): clear, colorless solution after reconstitution
Vial 2 (Minocyclinhydrochloride): clear, yellow solution after reconstitution
Vial 2 (Minocyclinhydrochloride): clear, yellow solution after reconstitution
Preparation Note
Working concentration: 10μg/ml pleuromutilin derivative (BM-cyclin 1), 5μg/ml tetracycline derivative (BM-cyclin 2).
Note: These concentrations do not affect the growth of most cells. Lower concentrations may be used for sensitive cell lines.
For hybridomas decrease concentration of BM-Cyclin by 50%.
Preparation of DAPI Working Solution
Dilute the stock solution with methanol to a final concentration of 1μg/ml.
Working solution: Preparation of Stock Solution
Dissolve content of BM Cyclin 1 and BM Cyclin 2 in 10ml sterile PBS or water.
Note: These are 250x concentrated stock solutions and can be stored at -15 to -25°C for at least 6 months.Preparation of DAPI Stock Solution
Dissolve DAPI in water to a final concentration of 1 to 5mg/ml.
Note: Do not use any buffers.
This stock solution can be stored at -15 to -25°C. It is recommended to prepare appropriate aliquots.
Storage conditions (working solution): -15 to -25°C
Reconstituted stable at -15 to -25°C for at least 6 months.
Note: These concentrations do not affect the growth of most cells. Lower concentrations may be used for sensitive cell lines.
For hybridomas decrease concentration of BM-Cyclin by 50%.
Preparation of DAPI Working Solution
Dilute the stock solution with methanol to a final concentration of 1μg/ml.
Working solution: Preparation of Stock Solution
Dissolve content of BM Cyclin 1 and BM Cyclin 2 in 10ml sterile PBS or water.
Note: These are 250x concentrated stock solutions and can be stored at -15 to -25°C for at least 6 months.Preparation of DAPI Stock Solution
Dissolve DAPI in water to a final concentration of 1 to 5mg/ml.
Note: Do not use any buffers.
This stock solution can be stored at -15 to -25°C. It is recommended to prepare appropriate aliquots.
Storage conditions (working solution): -15 to -25°C
Reconstituted stable at -15 to -25°C for at least 6 months.
Other Notes
For life science research only. Not for use in diagnostic procedures.
Kit Components Only
Product No.
Description
- BM-Cyclin 1: pleuromutilin derivative
- BM-Cyclin 2: tetracycline derivative
signalword
Warning
hcodes
Hazard Classifications
Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Storage Class
11 - Combustible Solids
wgk_germany
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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Mycoplasmas, the smallest and simplest prokaryotes that reside in endosomes of mammalian cells, are widespread contaminants found in cell cultures. About 30% of all cell cultures, varying from 15 to 80%, are reportedly contaminated with mycoplasmas. Here, we present our
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