SCT203
BioTracker™ Orange Zymosan Phagocytosis Live Cell Probe
Synonym(s):
Fluorescent Zymosan Probe, Live Cell Imaging Probe
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About This Item
Recommended Products
biological source
human
Quality Level
packaging
vial of 1 mg
manufacturer/tradename
Millipore
technique(s)
cell based assay: suitable
flow cytometry: suitable
shipped in
dry ice
storage temp.
−20°C
Application
"Zymosan A is a compound prepared from yeast cell walls and commonly used to induce inflammatory responses, especially phagocytosis. BioTracker™ Orange Zymosan Phagocytosis Live Cell Probe is Zymosan A labeled with an orange acidic pH indicator.
The fluorescence of this indicator is especially enhanced in an acidic environment such as that found in phagosomes or other acidic organelles. SCT203 is therefore a useful probe for the observaton of phagocytosis via its fluorescence increase, which can be only observed after phagocytosis. This probe emits fluorescence when excited by 532 or 514 nm sources, in addition to others compatible with the excitation range.
Spectral Properties
Absorbance (max): 535 nm
Emission (max): 568 nm (Orange)"
The fluorescence of this indicator is especially enhanced in an acidic environment such as that found in phagosomes or other acidic organelles. SCT203 is therefore a useful probe for the observaton of phagocytosis via its fluorescence increase, which can be only observed after phagocytosis. This probe emits fluorescence when excited by 532 or 514 nm sources, in addition to others compatible with the excitation range.
Spectral Properties
Absorbance (max): 535 nm
Emission (max): 568 nm (Orange)"
Features and Benefits
This live cell imaging probe is zymosan conjugated to fluorescent orange dye & can be used to detect phagocytosis using fluorescence microscopy or flow cytometry.
Storage and Stability
Store BioTracker™ Orange Zymosan Phagocytosis Live Cell Probe at -20°C, desiccated and protected from light. Centrifuge vial briefly to collect contents at bottom of vial before opening. Storing as a solution is not recommended.
Legal Information
BioTracker is a trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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