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Key Documents

SCT203

Sigma-Aldrich

BioTracker Orange Zymosan Phagocytosis Live Cell Probe

Synonym(s):

Fluorescent Zymosan Probe, Live Cell Imaging Probe

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About This Item

UNSPSC Code:
41106514

biological source

human

Quality Level

packaging

vial of 1 mg

manufacturer/tradename

Millipore

technique(s)

cell based assay: suitable
flow cytometry: suitable

shipped in

dry ice

storage temp.

−20°C

Application

"Zymosan A is a compound prepared from yeast cell walls and commonly used to induce inflammatory responses, especially phagocytosis. BioTracker Orange Zymosan Phagocytosis Live Cell Probe is Zymosan A labeled with an orange acidic pH indicator.

The fluorescence of this indicator is especially enhanced in an acidic environment such as that found in phagosomes or other acidic organelles. SCT203 is therefore a useful probe for the observaton of phagocytosis via its fluorescence increase, which can be only observed after phagocytosis. This probe emits fluorescence when excited by 532 or 514 nm sources, in addition to others compatible with the excitation range.

Spectral Properties
Absorbance (max): 535 nm
Emission (max): 568 nm (Orange)"

Features and Benefits

This live cell imaging probe is zymosan conjugated to fluorescent orange dye & can be used to detect phagocytosis using fluorescence microscopy or flow cytometry.

Storage and Stability

Store BioTracker Orange Zymosan Phagocytosis Live Cell Probe at -20°C, desiccated and protected from light. Centrifuge vial briefly to collect contents at bottom of vial before opening. Storing as a solution is not recommended.

Legal Information

BioTracker is a trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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