OP13
Anti-N-Myc Mouse mAb (NCM II 100)
liquid, clone NCM II 100, Calbiochem®
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.43
Recommended Products
biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
NCM II 100, monoclonal
form
liquid
does not contain
preservative
species reactivity
human, mouse
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
isotype
IgG1
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... MYCN(4613)
General description
Purified mouse monoclonal antibody generated by immunizing BALB/c mice with specified immunogen and fusing splenocytes with SP2/0 mouse myeloma cells (see application references). Recognizes N-myc and its cleavage products.
Recognizes the N-myc protein and its cleavage product in IMR5 cells.
This Anti-N-Myc Mouse mAb (NCM II 100) is validated for use in Frozen Sections, Immunoblotting, Immunofluorescence, Immunoprecipitation for the detection of N-Myc.
Immunogen
Human
recombinant, human, N-myc fusion protein, expressed in E. coli
Application
Frozen Sections (1-5 µg/ml)
Immunoblotting (1-5 µg/ml, see application references)
Immunofluorescence (1-5 µg/ml, see application references)
Immunoprecipitation (1-2 µg/sample, see application references)
Immunoblotting (1-5 µg/ml, see application references)
Immunofluorescence (1-5 µg/ml, see application references)
Immunoprecipitation (1-2 µg/sample, see application references)
Packaging
Please refer to vial label for lot-specific concentration.
Warning
Toxicity: Standard Handling (A)
Physical form
In 50 mM sodium phosphate buffer, 50% glycerol.
Reconstitution
Following initial thaw, aliquot and freeze (-20°C).
Analysis Note
Positive Control
IMR5 cells
IMR5 cells
Other Notes
Brondyk, W.H. 1991. Oncogene6, 1269.
Ikegaki, N., et al. 1988. Adv. Neuroblastoma Research.2, 133.
LeGouy, E., et al. 1987. In Nuclear Oncogenes, Cold Spring Harbor Laboratory, 144.
Cole, M.D., 1986. Ann. Rev. Gen.20, 361.
Ikegaki, N., et al. 1986. Proc. Natl. Acad. Sci. USA83, 5929.
Slamon, D.J., et al. 1986. Science232, 768.
Seeger, R.C., et al. 1985. New Engl. J. Med.313, 1111.
Brodeur, G.M., et al. 1984. Science224, 1121.
Michitsch, R.W., et al. 1984. Mol. Cell. Biol.11, 2370.
Kohl, N.E., et al. 1983. Cell35, 603.
Schwab, M., et al. 1983. Nature305, 245.
Ikegaki, N., et al. 1988. Adv. Neuroblastoma Research.2, 133.
LeGouy, E., et al. 1987. In Nuclear Oncogenes, Cold Spring Harbor Laboratory, 144.
Cole, M.D., 1986. Ann. Rev. Gen.20, 361.
Ikegaki, N., et al. 1986. Proc. Natl. Acad. Sci. USA83, 5929.
Slamon, D.J., et al. 1986. Science232, 768.
Seeger, R.C., et al. 1985. New Engl. J. Med.313, 1111.
Brodeur, G.M., et al. 1984. Science224, 1121.
Michitsch, R.W., et al. 1984. Mol. Cell. Biol.11, 2370.
Kohl, N.E., et al. 1983. Cell35, 603.
Schwab, M., et al. 1983. Nature305, 245.
It is important to compensate for the short half life of this protein when preparing tissue sections or extracts. We recommended that all preparations be kept cold and that a cocktail of protease inhibitors be used. Antibody should be titrated for optimal results in individual systems.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class
10 - Combustible liquids
wgk_germany
WGK 3
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Viktoryia Sidarovich et al.
Molecular biotechnology, 56(7), 631-643 (2014-02-12)
Neuroblastoma is the most common extracranial solid tumor of infancy. Amplification of MYCN oncogene is found in approximately 20 % of all neuroblastoma patients and correlates with advanced disease stages, rapid tumor progression, and poor prognosis, making this gene an
H P Wang et al.
Journal of virology, 72(3), 2192-2198 (1998-03-14)
Cell line WH44KA is a highly malignant woodchuck hepatoma cell line. WH44KA cells contain a single woodchuck hepatitis virus (WHV) DNA integration in the 3' untranslated region of exon 3 of the woodchuck N-myc1 gene. The highly rearranged WHV DNA
Erika A Newman et al.
Cell death & disease, 8(12), 3208-3208 (2017-12-15)
Neuroblastoma is a cancer of neural crest stem cell (NCSC) lineage. Signaling pathways that regulate NCSC differentiation have been implicated in neuroblastoma tumorigenesis. This is exemplified by MYCN oncogene targets that balance proliferation, differentiation, and cell death similarly in normal
James Chappell et al.
Genes & development, 27(7), 725-733 (2013-04-18)
Suppression of extracellular signal-regulated kinase (ERK) signaling is an absolute requirement for the maintenance of murine pluripotent stem cells (mPSCs) and requires the MYC-binding partner MAX. In this study, we define a mechanism for this by showing that MYC/MAX complexes
Giovanni Perini et al.
Proceedings of the National Academy of Sciences of the United States of America, 102(34), 12117-12122 (2005-08-12)
N-Myc is a transcription factor that forms heterodimers with the protein Max and binds gene promoters by recognizing a DNA sequence, CACGTG, called E-box. The identification of N-myc target genes is an important step for understanding N-Myc biological functions in
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