MABS2001M

Anti-LRP-1 Antibody, clone 11H4

clone 11H4, from mouse

• Synonym(s): Prolow-density lipoprotein receptor-related protein 1, Alpha-2-macroglobulin receptor1, A2MR, Apolipoprotein E receptor, APOER, CD91
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 11H4, monoclonal
• species reactivity: rat, mouse, human
• packaging: antibody small pack of 25 μg
• technique(s): immunocytochemistry: suitable; immunohistochemistry: suitable (paraffin); immunoprecipitation (IP): suitable; western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NP_002323
• UniProt accession no.: Q07954
• target post-translational modification: unmodified
• Gene Information: human ... LRP1(4035)

Description

General description

Prolow-density lipoprotein receptor-related protein 1 (UniProt: Q07954; also known as LRP-1, Alpha-2-macroglobulin receptor1, A2MR, Apolipoprotein E receptor, APOER, CD91) is encoded by the LRP1 (also known as A2MR, APR) gene (Gene ID: 4035) in human. LRP-1 is expressed in most tissues, but is abundant in liver, brain, and lung. LRP1 is synthesized with a signal peptide sequence (a.a. 1-19) and is processed in trans-Golgi network by furin to generate a 515 kDa alpha subunit and an 85 kDa beta subunit. The alpha and beta subunits are non-covalently linked during LRP1 transport to the cell membrane. LRP-1 recognizes and mediates the endocytosis of more than 40 different ligands, including apolipoprotein E (ApoE), APP and amyloid beta. It is the primary receptor mediating transport of amyloid beta peptides across the blood-brain barrier into circulation, thereby clearing them from the brain. LRP-1 is required for early embryonic development and is involved in cellular lipid homeostasis and plasma clearance of chylomicron remnants and activated LRPAP1 (alpha 2-macroglobulin). Excessive copper accumulation in the brain has been linked with reduced LRP-1 mediated clearance of amyloid beta peptides across the blood brain barrier, which may contribute to complications of Alzheimer′s disease.

Specificity

Clone 11H4 detects LRP-1 protein in human, mouse, and rat tissues. It targets an epitope within 13 amino acids from the C-terminal region.

Immunogen

KLH-conjugated linear peptide corresponding to 13 amino acid from the C-terminal region on human LRP-1.

Application

Anti-LRP-1, clone 11H4, Cat. No. MABS2001, is a mouse monoclonal antibody that detects Prolow-density lipoprotein receptor-related protein 1 in human, mouse, and rat and has been tested for use in Immunohistochemistry (Paraffin), Immunocytochemistry, Western Blotting and Immunoprecipitation.

Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected LRP-1 in human liver tissue.

Immunoprecipitation Analysis: A representative lot detected LRP-1 in Immunoprecipitation applications (Nieves, E.C., et. al. (2010). J Biol Chem. 285(17):12595-603).

Western Blotting Analysis: A representative lot detected LRP-1 in Western Blotting applications (Spoelgen, R., et. al. (2009). Neuroscience. 158(4):1460-8; Lleó, A., et. al. (2005). J Biol Chem. 280(29):27303-9; Campana, W.M., et. al. (2006). J Nuerosci. 26(43):11197-207).

Immunocytochemistry Analysis: A representative lot detected LRP-1 in Immunocytochemistry applications (Lleó, A., et. al. (2005). J Biol Chem. 280(29):27303-9).

Immunohistochemistry Analysis: A representative lot detected LRP-1 in Immunohistochemistry applications (Campana, W.M., et. al. (2006). J Nuerosci. 26(43):11197-207).

Research Category
Signaling

Quality

Evaluated by Immunohistochemistry in human brain tissue.

Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected LRP-1 in human brain tissue.

Target description

504.61 kDa calculated.

Physical form

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable