MABF233
Anti-HLA-C Antibody, clone DT9
clone DT9, from mouse
Synonym(s):
HLA class I histocompatibility antigen,Cw-1 alpha chain, MHC class I antigen Cw*1
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
DT9, monoclonal
species reactivity
human
technique(s)
flow cytometry: suitable
immunoprecipitation (IP): suitable
isotype
IgG2bκ
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... HLA-C(3107)
Related Categories
General description
HLA-C, also known as HLA class I histocompatibility antigen, Cw-1 alpha chain, also known as MHC class I antigen Cw*1, and encoded by the gene HLA-C/HLAC. It contains an alpha and beta (beta-2-microglobulin) chain and interacts with the human herpesvirus 8 MIR1 protein.
The previously assigned protein identifier P30499 has been merged into P10321. Full details can be found on the UniProt database.
The previously assigned protein identifier P30499 has been merged into P10321. Full details can be found on the UniProt database.
Immunogen
Purified tamarin MHC class I molecules corresponding to human HLA-C.
Application
Anti-HLA-C Antibody, clone DT9 is an antibody against HLA-C for use in flow cytometry & IP.
Flow Cytometry Analysis: A representative lot from an independent laboratory detected HLA-C in peripheral blood CD3+ cells from 200 healthy human donors (Apps, R., et al. (2013). Science. 340(6128):84-91.).
Flow Cytometry Analysis: A representative lot from an independent laboratory detected HLA-C in human PBMCs (Corrah, T. W., et al. (2011). J Virol. 85(7):3367-3374.).
Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated HLA-C from various human cell lines (Braud, V. M., et al. (1998). Curr Biol. 8(1):1-10.).
Flow Cytometry Analysis: A representative lot from an independent laboratory detected HLA-C in human PBMCs (Corrah, T. W., et al. (2011). J Virol. 85(7):3367-3374.).
Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated HLA-C from various human cell lines (Braud, V. M., et al. (1998). Curr Biol. 8(1):1-10.).
Research Category
Inflammation & Immunology
Inflammation & Immunology
Research Sub Category
Inflammation & Autoimmune Mechanisms
Inflammation & Autoimmune Mechanisms
Quality
Evaluated by Flow Cytometry in human PBMCs.
Flow Cytometry Analysis: 1 µg of this antibody detected HLA-C in 1X10E6 human PBMCs.
Flow Cytometry Analysis: 1 µg of this antibody detected HLA-C in 1X10E6 human PBMCs.
Target description
41 kDa calculated
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Tumena W Corrah et al.
Journal of virology, 85(7), 3367-3374 (2011-01-21)
Previous studies have found an association between a single-nucleotide polymorphism 35 kb upstream of the HLA-C locus (-35 SNP), HLA-C expression, and HIV-1 set point viral loads. We show that the difference in HLA-C expression across -35 SNP genotypes can
V M Braud et al.
Current biology : CB, 8(1), 1-10 (1998-03-28)
The human major histocompatibility complex (MHC) class lb molecule HLA-E is transcribed in most tissues but little is known about its localisation within the cell. We have recently shown that HLA-E binds signal-sequence-derived peptides from human MHC class I molecules
Kristina Hopfensperger et al.
mBio, 11(4) (2020-07-16)
HLA-C-mediated antigen presentation induces the killing of human immunodeficiency virus (HIV)-infected CD4+ T cells by cytotoxic T lymphocytes (CTLs). To evade killing, many HIV-1 group M strains decrease HLA-C surface levels using their accessory protein Vpu. However, some HIV-1 group
Richard Apps et al.
Science (New York, N.Y.), 340(6128), 87-91 (2013-04-06)
A variant upstream of human leukocyte antigen C (HLA-C) shows the most significant genome-wide effect on HIV control in European Americans and is also associated with the level of HLA-C expression. We characterized the differential cell surface expression levels of
Eri Yamada et al.
Cell host & microbe, 23(1), 110-120 (2018-01-13)
The HIV-1-encoded accessory protein Vpu exerts several immunomodulatory functions, including counteraction of the host restriction factor tetherin, downmodulation of CD4, and inhibition of NF-κB activity to facilitate HIV-1 infection. However, the relative contribution of individual Vpu functions to HIV-1 infection
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