MilliporeSigma
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11363735001

Roche

Hybridoma Fusion and Cloning Supplement

solution, suitable for hybridoma, Mycoplasma, tested

Synonym(s):
HFCS

Quality Level

description

HFCS

sterility

0.2 μm filtered

form

solution

packaging

pkg of 10 mL (50x)

manufacturer/tradename

Roche

technique(s)

cell culture | hybridoma: suitable

impurities

Mycoplasma, tested
10 EU/mL Endotoxin (LAL)

solubility

water: miscible

shipped in

dry ice

storage temp.

−20°C

General description

Medium supplement composed of albumin, insulin, transferrin, cytokines, a cholesterol source, other defined organic and inorganic compounds and fetal calf serum (final concentration: 0.5%).
HFCS contains human proteins. The raw material from which the human proteins were isolated, has been tested for the presence of Hepatitis B surface Antigen (HBsAg) and HIV-1/2 antibodies and found to be negative (according to current quality control procedures).

Application

Hybridoma Fusion and Cloning Supplement (HFCS) is used as a supplement to the normal culture medium to support the growth of B-cell hybridomas after fusion and during cloning. HFCS replaces feeder cells. It is also used to optimize the growth of hybridomas after the thawing of cells stored in liquid nitrogen.

Physical form

Solution (50x concentrated, pH 7.4); filtered through 0.2 μm pore size membrane

Preparation Note

Working concentration: HFCS (50x) is diluted 1:50 (v/v) with basal medium. It is strongly suggested that you use RPMI 1640. The final medium should also contain L-glutamine and sodium bicarbonate.

Analysis Note

Biological activity: Assayed for high cloning efficiency of a hybridoma cell line.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

does not flash

Flash Point(C)

does not flash

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Product Information Sheet

Quotes and Ordering

Toshiyuki Sasaki et al.
International journal of cancer, 134(7), 1558-1570 (2013-10-10)
Phenotypic expression of human leukocyte antigen (HLA)-E on the surface of tumor lesions includes intact heterodimer [HLA-E heavy chain and β2-microglobulin (β2m)] and β2m-free monomer. Anti-HLA-E monoclonal antibodies (mAbs), MEM-E/02 or 3D12 bind to the peptide sequences in β2m-free HLA-E
Mepur H Ravindranath et al.
Blood, 121(11), 2013-2028 (2013-01-12)
The US Food and Drug Administration approved intravenous immunoglobulin (IVIg), extracted from the plasma of thousands of blood donors, for removing HLA antibodies (Abs) in highly sensitized patients awaiting organ transplants. Since the blood of healthy individuals has HLA Abs

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