MAB4399-I
Anti-CD133 (Prominin-1) Antibody, clone 17A6.1
clone 17A6.1, from mouse
Synonym(s):
Prominin-1, Prominin-like protein 1, Antigen CD133
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
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biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
17A6.1, monoclonal
species reactivity
human
technique(s)
immunocytochemistry: suitable
western blot: suitable
isotype
IgG2aκ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... PROM1(8842)
General description
Prominin-1 (UniProt: O43490; also known as Antigen AC133, Prominin-like protein 1, CD133) is encoded by the PROM1 (also known as PROML1, MSTP061) gene (Gene ID: 8842) in human. CD133 (Prominin-1) is multi-pass cell-surface glycoprotein that plays a role in cell differentiation, proliferation and apoptosis. It binds cholesterol in cholesterol-containing plasma membrane microdomains and may play a role in the organization of the apical plasma membrane in epithelial cells. During early retinal development CD133 acts as a key regulator of disk morphogenesis. Defects in PROM1 gene are known to cause retinitis pigmentosa, cone-rod dystrophy, and retinal macular dystrophy 2. Higher levels of CD133 have been reported in several cancer cell types, including various leukemias, retinoblastomas, glioblastomas, and kidney carcinomas. Higher levels of CD133 are also observed in pancreatic, gastric, colorectal, and hepatocellular cancers. It is also considered as a marker of stem cells in the adult small intestine that are susceptible to transformation into tumors retaining a fraction of mutant Prom1+ tumor cells. (Ref.: Zhu, L., et al. (2009). Nature 477 (7229); 603-607).
Specificity
Clone 17A6.1 detects recombinant CD133 (Prominin-1) by Western blotting and also detects CD133 (Prominin-1 ) in U251 cells by immunocytochemistry.
Immunogen
GST-tagged recombinat fragment corresponding to 77 amino acids from the N-terminal region of human CD133 (Prominin-1).
Application
Detect CD133 using this mouse monoclonal Anti-CD133 (Prominin-1) antibody, clone 17A6.1, Cat. No. MAB4399-I. Validated for use in Immunocytochemistry.
Immunocytochemistry Analysis: A 1:50 dilution from a representative lot detected CD133 in U251 cells.
Quality
Evaluated by Western Blotting with recombinant Prominin-1.
Western Blotting Analysis: 1 µg/mL of this antibody detected 2.5 µg of recombinant Prominin-1 protein.
Western Blotting Analysis: 1 µg/mL of this antibody detected 2.5 µg of recombinant Prominin-1 protein.
Target description
~97 kDa observed; 97.2 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physical form
Format: Purified
Other Notes
Concentration: Please refer to lot specific datasheet.
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recommended
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
Certificates of Analysis (COA)
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Cong Li et al.
Oncology letters, 22(1), 499-499 (2021-05-14)
Cancer stem cells (CSCs) and epithelial mesenchymal transition (EMT) markers are considered useful indicators associated with metastasis and prognosis of colorectal cancers (CRCs). However, only a few studies have focused on the expression of these useful markers in metastases. Metastasectomy
Yi Sui et al.
British journal of cancer, 125(7), 1003-1015 (2021-07-12)
Metastasis is the major cause of treatment failure and cancer-related deaths in prostate cancer (PCa) patients. Our previous study demonstrated that a CD44+ subpopulation isolated from PCa cells or tumours possesses both stem cell properties and metastatic potential, serving as
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