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MAB4381C3

Sigma-Aldrich

Anti-TRA-1-81 Antibody, clone TRA-1-81, Cy3 conjugate

clone TRA-1-81, from mouse, CY3 conjugate

Synonym(s):

Podocalyxin, GCTM-2 antigen, Gp200, Podocalyxin-like protein 1

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

conjugate

CY3 conjugate

antibody form

saturated ammonium sulfate (SAS) precipitated

antibody product type

primary antibodies

clone

TRA-1-81, monoclonal

species reactivity

human

technique(s)

immunocytochemistry: suitable

isotype

IgM

NCBI accession no.

NP_001018121.1

UniProt accession no.

O00592

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... PODXL(5420)

General description

TRA-1-81 is expressed upon the surface of human teratocarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES). Both TRA-1-81 and TRA-1-60 recognize antigens that are associated with a pericellular matrix proteoglycan. TRA-1-60 reacts with a sialidase-sensitive epitope while TRA-1-81 reacts with an unknown epitope of the same molecule. TRA-1-81 is commonly used as marker of undifferentiated pluripotent human stem cells and is downregulated upon differentiation. TRA-1-81 has recently been identified as podocalyxin.

Specificity

This antibody recognizes human TRA-1-81.

Immunogen

Human embryonal carcinoma cell line 2102Ep

Application

Evaluated by Immunocytochemistry in mouse embryonic stem cells (SCR012). Immunocytochemsitry Analysis: A 1:100 dilution of this antibody did not detect TRA-1-81 in mouse embryonic stem cells (SCR012).
Research Category
Stem Cell Research
Research Sub Category
Pluripotent & Early Differentiation
This Anti-TRA-1-81 Antibody, clone TRA-1-81, Cy3 conjugate is validated for use in ICC for the detection of TRA-1-81.

Quality

Evaluated by Immunocytochemistry in H9 human embryonic stem cells.
Immunocytochemsitry Analysis: A 1:100 dilution of this antibody detected TRA-1-81 in H9 human embryonic stem cells.

Target description

420/250 kDa calculated

Physical form

Purified mouse monoclonal IgM conjugated to Cy3 in PBS with 0.1% sodium azide and 15mg/ml BSA.

Storage and Stability

Maintain refrigerated at 2-8ºC protected from light in undiluted aliquots for up to 6 months from date of receipt.

Analysis Note

Control
H9 human embryonic stem cells

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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A nonviral minicircle vector for deriving human iPS cells.
Jia, Fangjun, et al.
Nature Methods, 7, 197-199 (2010)
G Badcock et al.
Cancer research, 59(18), 4715-4719 (1999-09-24)
Human embryonal carcinoma (EC) cells are the stem cells of teratocarcinomas, and they are key components of germ cell tumors (GCTs). They express several high molecular weight glycoprotein antigens that are down-regulated upon differentiation. One of these antigens, defined by
Three monoclonal antibodies defining distinct differentiation antigens associated with different high molecular weight polypeptides on the surface of human embryonal carcinoma cells.
Andrews, P W, et al.
Hybridoma, 3, 347-361 (1984)
Ignasi Rodríguez-Pizà et al.
Stem cells (Dayton, Ohio), 28(1), 36-44 (2009-11-06)
The availability of induced pluripotent stem cells (iPSCs) has created extraordinary opportunities for modeling and perhaps treating human disease. However, all reprogramming protocols used to date involve the use of products of animal origin. Here, we set out to develop
Athurva Gore et al.
Nature, 471(7336), 63-67 (2011-03-04)
Defined transcription factors can induce epigenetic reprogramming of adult mammalian cells into induced pluripotent stem cells. Although DNA factors are integrated during some reprogramming methods, it is unknown whether the genome remains unchanged at the single nucleotide level. Here we
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