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MAB2053

Sigma-Aldrich

Anti-Disialoganglioside GD3 Antibody, clone MB3.6

clone MB3.6, Chemicon®, from mouse

Synonym(s):

GD3 Antibody

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

MB3.6, monoclonal

species reactivity

human

manufacturer/tradename

Chemicon®

technique(s)

flow cytometry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable

isotype

IgG3

shipped in

wet ice

target post-translational modification

unmodified

Related Categories

Specificity

Reacts specifically with human GD3 ganglioside (Cheresh et al., 1984). No cross-reactivity with other gangliosides purified from neuroblastoma or melanoma cells.

Immunogen

FM9 melanoma cells

Application

Anti-Disialoganglioside GD3 Antibody, clone MB3.6 is an antibody against Disialoganglioside GD3 for use in FC, IF, IH.
Immunohistochemistry (frozen tissue sections)

Immunofluorescence of cells in culture

Flow cytometry: 1-2 μg per 10E6 cells

Lysis of GD3 positive (melanoma) cells in presence of complement (Cheresh et al., 1985).

Induction of antibody dependent cellular cyotoxicity (ADCC) {Ortaldo JR et al 1987 J Immunol 138(10):3566-3572.

TLC analysis: Sjoberg, ER (1995) J. Biol. Chem. 270(7):2921-2930.{http://www.jbc.org/cgi/content/full/270/7/2921}





Optimal working dilutions must be determined by end user.

Physical form

Format: Purified
Liquid in 0.02M PB pH 7.6, 0.25M NaCl containing 0.1% NaN3.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Characterization of new serum biomarkers in breast cancer using lipid microarrays.
Yoshiya Yonekubo,Ping Wu,Aimalohi Esechie,Yueqiang Zhang,Guangwei Du
Tumour Biology : the Journal of the International Society For Oncodevelopmental Biology and Medicine null
Analysis of effector cells in human antibody-dependent cellular cytotoxicity with murine monoclonal antibodies.
Ortaldo, JR et al.
Journal of immunology (Baltimore, Md. : 1950), 138(10), 3566-3572 (1987)
Biosynthesis and expression of the disialoganglioside GD2, a relevant target antigen on small cell lung carcinoma for monoclonal antibody-mediated cytolysis.
Cheresh, D A, et al.
Cancer Research, 46, 5112-5118 (1986)
Suzanne M Birks et al.
Neuro-oncology, 13(9), 950-960 (2011-08-03)
The expression of ganglioside GD3, which plays crucial roles in normal brain development, decreases in adults but is upregulated in neoplastic cells, where it regulates tumor invasion and survival. Normally a buildup of GD3 induces apoptosis, but this does not

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