MABC1112
Anti-Diasialoganglioside GD3 Antibody, clone R24
clone R24, from mouse
Synonym(s):
GD3, Ganglioside GD3, Disialoganglioside GD3
About This Item
biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
R24, monoclonal
species reactivity
human, mouse, rat
technique(s)
affinity binding assay: suitable
flow cytometry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG3κ
shipped in
ambient
target post-translational modification
unmodified
Gene Information
human ... ST8SIA1(6489)
General description
Specificity
Immunogen
Application
Affinity Binding Assay: A representative lot reacted with heat stable glycolipid on the surface of all SK-MEL melanoma and two of the five astrocytoma cells tested, whereas epithelial cell types, fibroblasts, and cells of hematopoietic origin were found to lack the surface antigen recognized by clone R24 (Dippold, W.G., et al. (1980). Proc. Natl. Acad. Sci. U.S.A. 77(10):6114-6118).
Flow Cytometry Analysis: A representative lot immunostained the surface of cultured primary rat cerebellar cells (Kasahara, K., et al. (1997). J. Biol. Chem. 272(47):29947-29953).
Immunocytochemistry Analysis: A representative lot immunostained NGF-induced nurite outgrowths of cultured DRG neurons from wild-type, but not GD3 synthase-knockout, mice (Ribeiro-Resende, V.T., et al. (2014). PLoS One. 9(10):e108919).
Immunohistochemistry Analysis: A representative lot detected GD3 immunoreactivity in unfixed frozen tissue samples of primary melanoma and metastatic malignant melanoma (Dippold, W.G., et al. (1985). Cancer Res. 45(8):3699-3705).
Immunoprecipitation Analysis: A representative lot co-immunoprecipitated Lyn from rat brain membrane extract. Clone R24 co-immunoprecipitated caveolin and the exogenously expressed Lyn, but not Src, from CHO cells expressing exogenous GD3 synthase (Kasahara, K., et al. (1997). J. Biol. Chem. 272(47):29947-29953).
Apoptosis & Cancer
Quality
Flow Cytometry Analysis: 0.2 µL of this antibody detected GD3 immunoreactivity on the surface of one million SK-MEL-28 human skin melanoma cells.
Physical form
Storage and Stability
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Other Notes
Disclaimer
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Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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