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biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
3E1, monoclonal
mol wt
calculated mol wt 202.17 kDa
species reactivity
human
packaging
antibody small pack of 100 μL
technique(s)
ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
2-8°C
target post-translational modification
unmodified
Gene Information
human ... ITGB4(3691)
General description
Integrin β4 (UniProt: P16144; also known as GP150, CD104) is encoded by the ITGB4 gene (Gene ID: 3691) in human. Integrins are heterodimeric cell surface receptors composed of a variable alpha subunit and a conserved beta subunit. The exact combination of various alpha and beta subunits dictates the binding specificity of integrins to different ECM components. Integrins are not constitutively active. Activation of an integrin from low ligand-binding affinity state to high ligand-binding affinity state requires conformational change that can originate from either from their cytoplasmic or extracellular interactions. The recognition site for most integrins that bind the ECM consists of an RGD (arginine-glycine-aspartic acid) sequence. Integrins bind to their ligands with low affinity and this binding occurs only when a certain minimum number of integrins are present at specific points known as focal contacts. Integrin β4 is a single-pass type I membrane protein that is synthesized with a signal peptide (aa 1-27), which is subsequently cleaved off to generate the mature form that contains an extracellular domain (aa 28-710), a transmembrane domain (aa 711-733), and a cytoplasmic domain (aa 734-1822). It contains four fibronectin type III domains. Integrin β4 heterodimerizes with a6 that serves as a receptor for laminin and plays a critical structural role in the hemidesmosome of epithelial cells. It undergoes palmitoylation by DHHC3 at several cysteines of the membrane-proximal region that enhances its stability and surface expression. Palmitoylation also promotes its secondary association with tetraspanins.
Specificity
Clone 3E1 is a mouse monoclonal antibody that detects Integrin β4. It targets an epitope within the extracellular domain.
Immunogen
Human fetal cell membranes.
Application
Quality Control Testing
Evaluated by Immunocytochemistry in A431 cells.
Immunocytochemistry Analysis: A 1:1,000 dilution of this antibody detected Integrin β4 in A431 cells.
Tested Applications
Flow Cytometry Analysis: 1 βg from a representative lot detected Integrin β4 in one million A431 cells.
Flow Cytometry Analysis: A representative lot detected Integrin β4 in Flow Cytometry applications (Vijayakumar, S., et. al. (2008). J. Am. Soc. Nephrol.19(6):1079-91; Hauptmann, S., et. al. (1995). Int. J. Cancer. 61(6):819-25).
Immunohistochemistry Applications: A representative lot detected Integrin β4 in Immunohistochemistry applications ( Hauptmann, S., et. al. (1995). Int. J. Cancer. 61(6):819-25).
Immunofluorescence Analysis: A representative lot detected Integrin β4 in Immunofluorescence applications (Swanner, J., et. al. (2019). FASEB BioAdv. 1(10):639-660).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Immunocytochemistry in A431 cells.
Immunocytochemistry Analysis: A 1:1,000 dilution of this antibody detected Integrin β4 in A431 cells.
Tested Applications
Flow Cytometry Analysis: 1 βg from a representative lot detected Integrin β4 in one million A431 cells.
Flow Cytometry Analysis: A representative lot detected Integrin β4 in Flow Cytometry applications (Vijayakumar, S., et. al. (2008). J. Am. Soc. Nephrol.19(6):1079-91; Hauptmann, S., et. al. (1995). Int. J. Cancer. 61(6):819-25).
Immunohistochemistry Applications: A representative lot detected Integrin β4 in Immunohistochemistry applications ( Hauptmann, S., et. al. (1995). Int. J. Cancer. 61(6):819-25).
Immunofluorescence Analysis: A representative lot detected Integrin β4 in Immunofluorescence applications (Swanner, J., et. al. (2019). FASEB BioAdv. 1(10):639-660).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-Integrin β4, clone 3E1, Cat. No. MAB1964-I, is a mouse monoclonal antibody that detects Integrin β4 and is tested for use in Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry.
Physical form
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at +2°C to +8°C from date of receipt.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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