ECM205
Millicoat® ECM Screening Kit, 1 ea. ECM101-ECM105
Millicoat®, pkg of 96-well plate(s) (for fibronectin, vitronectin, laminin, collagen I & collagen IV)
Synonym(s):
Formerly under the CytoMatrix brand name.
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About This Item
UNSPSC Code:
12352202
eCl@ss:
32161000
NACRES:
NA.77
Recommended Products
species reactivity
human
Quality Level
manufacturer/tradename
Chemicon®
Millicoat®
packaging
pkg of 96-well plate(s) (for fibronectin, vitronectin, laminin, collagen I & collagen IV)
technique(s)
activity assay: suitable
cell based assay: suitable
input
sample type neural stem cell(s)
sample type: human embryonic stem cell(s)
sample type: mouse embryonic stem cell(s)
sample type epithelial cells
sample type hematopoietic stem cell(s)
sample type pancreatic stem cell(s)
sample type induced pluripotent stem cell(s)
sample type mesenchymal stem cell(s)
detection method
colorimetric
shipped in
wet ice
General description
Cell adhesion plays a major role in cellular communication and regulation, and is of fundamental importance in the development and maintenance of tissues. Scientists are continually examining the adhesion and migration of many diverse cell types on various extracellular matrix (ECM) component proteins. Millicoat® Cell Adhesion Strips are provided as 12 8-well removable strips in a plate frame for convenience and flexibility in designing assays. The wells in rows A - G have been coated with a human ECM protein. Row H of each strip is coated with BSA which serves as a negative assay control. The Millicoat screening kit contains individual 96-well plates for fibronectin, vitronectin, laminin, collagen I and collagen IV. Cells are seeded onto the coated substrate. Subsequently, adherent cells are fixed and stained. Relative attachment is determined using absorbance readings.
Application
PROCEDURE:
NOTE: Optimal assay performance is obtained using subconfluent cell cultures. This can be achieved by splitting the cells 1 to 2 days prior to performing the assay.
1. Rehydrate the strips with 200 mL of PBS per well for at least 15 minutes at room temperature. Remove the PBS from the rehydated strips.
2. Prepare a single cell suspension, preferably using a non-enzymatic dissociation buffer. Optimum cell density may be determined by titration of the cells. A common starting range is between 1x10E05 to 1x10E07 cells/mL.
3. Add 100 mL of the diluted cell suspension to each well. Incubate the plate at 37°C for 1 hour in a CO2 incubator. Gently wash the plate 3 times with PBS containing Ca2+/Mg2+ (200 mL/well).
4. Add 100 mL/well of 0.2% crystal violet in 10% ethanol to each well. Incubate for 5 minutes at room temperature. Remove the stain from the wells. Gently wash the plate 3 times with PBS (300 mL/well).
5. Add 100 mL of Solubilization Buffer (A 50/50 mixture of 0.1M NaH2PO, pH 4.5 and 50% ethanol) to each well. Allow strips to incubate and gently shake at room temperature until the cell-bound stain is completely solubilized; approximately 5 minutes.
6. Determine the absorbances at 540 - 570 nm on a microplate reader.
NOTE: Optimal assay performance is obtained using subconfluent cell cultures. This can be achieved by splitting the cells 1 to 2 days prior to performing the assay.
1. Rehydrate the strips with 200 mL of PBS per well for at least 15 minutes at room temperature. Remove the PBS from the rehydated strips.
2. Prepare a single cell suspension, preferably using a non-enzymatic dissociation buffer. Optimum cell density may be determined by titration of the cells. A common starting range is between 1x10E05 to 1x10E07 cells/mL.
3. Add 100 mL of the diluted cell suspension to each well. Incubate the plate at 37°C for 1 hour in a CO2 incubator. Gently wash the plate 3 times with PBS containing Ca2+/Mg2+ (200 mL/well).
4. Add 100 mL/well of 0.2% crystal violet in 10% ethanol to each well. Incubate for 5 minutes at room temperature. Remove the stain from the wells. Gently wash the plate 3 times with PBS (300 mL/well).
5. Add 100 mL of Solubilization Buffer (A 50/50 mixture of 0.1M NaH2PO, pH 4.5 and 50% ethanol) to each well. Allow strips to incubate and gently shake at room temperature until the cell-bound stain is completely solubilized; approximately 5 minutes.
6. Determine the absorbances at 540 - 570 nm on a microplate reader.
Research Category
Cell Structure
Cell Structure
The Millicoat® screening kit contains individual 96-well plates for fibronectin, vitronectin, laminin, collagen I & collagen IV.
Storage and Stability
May be stored at 2-8°C in the foil pouch for at least 3 months. Unused strips may be placed back in the pouch for storage. Ensure that the desiccant remains in the pouch, and that the pouch is securely closed.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
MILLICOAT is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class
11 - Combustible Solids
Certificates of Analysis (COA)
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Articles
Extracellular matrix proteins such as laminin, collagen, and fibronectin can be used as cell attachment substrates in cell culture.
Protocols
This page covers the ECM coating protocols developed for four types of ECMs on Millicell®-CM inserts, Collagen Type 1, Fibronectin, Laminin, and Matrigel.
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