ABD121
Anti-PRDM14 Antibody
from rabbit
Synonym(s):
PRDM-14, PR domain zinc finger protein 14
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
unpurified
antibody product type
primary antibodies
clone
polyclonal
species reactivity
human
technique(s)
ChIP: suitable (ChIP-seq)
western blot: suitable
isotype
IgG
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... PRDM14(63978)
General description
PRDM14 is an approximately 65 kDa transcriptional inhibitor that contains one histone methylating PR/SET domain (aa 266 - 367) and six zinc finger repeats (aa 400 - 568). PRDM14 is preferentially expressed in germ cells and undifferentiated embryonic stem cells in which it plays a central role in lineage specification, maintenance of pluripotency, and repression of somatic gene transcription. PRDM14 is upregulated in breast cancer and lymphoblastic leukemia. Recently, PRDM14 ensures naive pluripotency through dual regulation of signaling and epigenetic pathways in embryonic stem cells
Specificity
Human
Immunogen
Epitope: N-terminus
Human recombinant PRDM14.
Application
Chromatin Immunoprecipitation Analysis: A representative lot was used by an independent laboratory on H1 human ES cells (CHAN et al., 2013. STEM CELLS, 31:682–692)
ChIPseq Analysis: A representative lot was used by an independent laboratory on H1 human ES cells (Chia et al., 2010. Nature. 316, VOL 468)
ChIPseq Analysis: A representative lot was used by an independent laboratory on H1 human ES cells (Chia et al., 2010. Nature. 316, VOL 468)
Research Category
Stem Cell Research
Stem Cell Research
Research Sub Category
Pluripotent & Early Differentiation
Pluripotent & Early Differentiation
This Anti-PRDM14 Antibody is validated for use in Western Blotting and Chromatin Immunoprecipitation (ChIP) and ChIP-seq for the detection of PRDM14 .
Quality
Evaluated by Western Blotting in Human ES cell cell lysate. Western Blot Analysis: 1 µg/mL of this antibody detected PRDM14 in undifferentiated human ES cell lysate.
Target description
~64 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Physical form
Format: Unpurified
Raw rabbit polyclonal serum
Unpurified
Storage and Stability
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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A PRC2-dependent repressive role of PRDM14 in human embryonic stem cells and induced pluripotent stem cell reprogramming.
Chan, Yun-Shen, et al.
Stem Cells, 31, 682-692 (2013)
Na-Yu Chia et al.
Nature, 468(7321), 316-320 (2010-10-19)
The derivation of human ES cells (hESCs) from human blastocysts represents one of the milestones in stem cell biology. The full potential of hESCs in research and clinical applications requires a detailed understanding of the genetic network that governs the
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