AB3562P
Anti-Na+/K+/Cl- Cotransporter 2 Antibody
Chemicon®, from rabbit
Synonym(s):
NKCC2
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
affinity purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
mouse, rat
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... SLC12A1(6557)
Specificity
Recognizes rat Sodium, Potassium, Chloride Cotransporter 2 (NKCC2, BSC1, CCC2). The immunogen shows no significant sequence homology with NKCC1 or TSC.
SPECIES REACTIVITIES: The immunogen sequence is 100% conserved in mouse, 87% in human and rabbit. Reactivity with other species has not been confirmed.
SPECIES REACTIVITIES: The immunogen sequence is 100% conserved in mouse, 87% in human and rabbit. Reactivity with other species has not been confirmed.
Immunogen
A 15 amino acid peptide sequence near the C-terminus of rat NKCC2 (Haas et al. 1987; Gamba et al. 1994; Simon et al. 1996; Mount et al. 1995; Igarashi et al. 1995; Mount et al. 1998). The immunogen peptide is available (catalog number AG205) for use in pre-absortion controls.
Application
Anti-Na+/K+/Cl-Cotransporter 2 Antibody detects level of Na+/K+/Cl-Cotransporter 2 & has been published & validated for use in ELISA & WB.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Ion Channels & Transporters
Ion Channels & Transporters
Western blot: 1-10 μg/mL using ECL. Full length human NKCC2 is ~150 kDa.
ELISA: 0.5-1 μg/mL using 1 μg/mL control peptide per well.
Optimal working dilutions must be determined by the end user.
ELISA: 0.5-1 μg/mL using 1 μg/mL control peptide per well.
Optimal working dilutions must be determined by the end user.
Physical form
Affinity purified immunoglobulin. Liquid in PBS containing 0.1% BSA.
Storage and Stability
Maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Dendritic compartmentalization of chloride cotransporters underlies directional responses of starburst amacrine cells in retina.
Gavrikov, KE; Nilson, JE; Dmitriev, AV; Zucker, CL; Mangel, SC
Proceedings of the National Academy of Sciences of the USA null
Determination of uromodulin in human urine: influence of storage and processing.
Youhanna, S; Weber, J; Beaujean, V; Glaudemans, B; Sobek, J; Devuyst, O
Nephrology, Dialysis, and Transplantation null
Ting Li et al.
Molecular medicine reports, 17(6), 7618-7626 (2018-04-06)
Acute kidney injury (AKI) is one of the most common complications of sepsis. The roles of autophagy in AKI have been demonstrated in previous studies. Sequestosome‑1 (p62) has been demonstrated to serve essential roles in autophagy. The dysregulation of autophagy
Katharina Gerl et al.
The American journal of pathology, 187(7), 1496-1511 (2017-05-21)
Intrauterine hypoxia is a reason for impaired kidney development. The cellular and molecular pathways along which hypoxia exerts effects on nephrogenesis are not well understood. They are likely triggered by hypoxia-inducible transcription factors (HIFs), and their effects appear to be
Agnieszka Konopacka et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 35(13), 5144-5155 (2015-04-04)
The Na-K-2Cl cotransporter 2 (NKCC2) was thought to be kidney specific. Here we show expression in the brain hypothalamo-neurohypophyseal system (HNS), wherein upregulation follows osmotic stress. The HNS controls osmotic stability through the synthesis and release of the neuropeptide hormone
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