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Key Documents

AB2231

Sigma-Aldrich

Anti-Dopamine Transporter; 100 µg Antibody

from rabbit

Synonym(s):

DA transporter, dopamine transporter, solute carrier family 6 (neurotransmitter transporter, dopamine),member 3, variable number tandem repeat (VNTR)

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

species reactivity

mouse, rat

technique(s)

western blot: suitable

NCBI accession no.

NM_001044.3

UniProt accession no.

Q01959

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

mouse ... Slc6A3(13162)
rat ... Slc6A3(24898)

General description

Plasmalemmal neurotransmitter transporters sequester synaptic and peri synaptic transmitter into presynaptic elements. The Dopamine Transporter (DAT), a 12 transmembrane domain transporter with the N and C terminal regions located within the cytoplasm is responsible for the reaccumulation of dopamine after it has been released. Levels of DAT protein expression are altered by chronic drug administration. The activity of the DAT reuptake carrier is sodium dependent, and it is suspected to play a role in such neurologic and psychiatric disorders as Parkinson′s disease, Tourette′s disease, schizophrenia, and addiction. DAT is also the target of several “DAT-blockers” including amphetamines and cocaine. These chemicals inhibit the action of DAT and, to a lesser extent, the other monoamine transporters, but their effects are mediated by separate mechanisms.

Specificity

Cat. AB2231 recognizes the N-terminus of the reduced and non reduced forms of DAT.

Application

This Anti-Dopamine Transporter, 100 µg Antibody is validated for use in WB for the detection of Dopamine Transporter, 100 µg.

Quality

Routinelyevaluated by western blot on rat brain lysate.

Target description

~ 50 – 80 kDa
Reduced form at approx 50 kDa and Non-reduced form at approx. 80 kDa

Physical form

Format: Purified

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Dafina M Angelova et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 32(2), 995-1006 (2017-10-22)
α-Synuclein (α-syn) is associated with a range of diseases, including Parkinson disease. In disease, α-syn is known to aggregate and has the potential to be neurotoxic. The association between copper and α-syn results in the formation of stellate toxic oligomers
Daniel W Curry et al.
Neuropharmacology, 128, 196-206 (2017-10-11)
S,R(+/-)-3,4-methylenedioxymethamphetamine (SR-MDMA) is an amphetamine derivative with prosocial and putative therapeutic effects. Ongoing clinical trials are investigating it as a treatment for post-traumatic stress disorder (PTSD) and other conditions. However, its potential for adverse effects such as hyperthermia and neurotoxicity
Traumatic brain injury and trichloroethylene exposure interact and produce functional, histological, and mitochondrial deficits.
Sauerbeck, A; Hunter, R; Bing, G; Sullivan, PG
Experimental neurology null
M Y Chang et al.
Journal of neurochemistry, 77(3), 754-761 (2001-05-02)
Dopaminergic neurotransmission is terminated by the action of the presynaptic dopamine transporter (DAT). It mediates Na(+)/Cl(-) -dependent re-uptake of extracellular dopamine (DA) into the cell, and is regarded as a major regulatory mechanism for synaptic transmission. Previous works have documented
Symmetrical dimer of the human dopamine transporter revealed by cross-linking Cys-306 at the extracellular end of the sixth transmembrane segment.
Hastrup, H, et al.
Proceedings of the National Academy of Sciences of the USA, 98, 10055-10060 (2001)
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