70856
RNase A Solution
Convenient solution for selective degradation of RNA
Synonym(s):
RNase A Enzyme
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About This Item
UNSPSC Code:
12352202
NACRES:
NA.54
form
solution
manufacturer/tradename
Novagen®
storage condition
OK to freeze
concentration
10 mg/mL
technique(s)
DNA purification: suitable
shipped in
ambient
storage temp.
10-30°C
General description
RNase A Solution is a convenient alternative to powdered RNase A. It is a highly purified preparation of bovine pancreatic ribonuclease A suitable for use in selective removal of RNA. It has been pretreated to remove DNase I and is suitable to remove RNA contamination during plasmid and genomic DNA purification procedures. Supplied at a concentration of 10 mg/ml in 10 mM Tris-HCl, 1 mM EDTA, 50% glycerol, pH 7.5.
Application
RNase A Solution has been used:
- in cell cycle profiling of embryonic mouse neuroectodermal cells (NE-4C)
- in the digestion of RNA for extraction of DNA from human osteosarcoma cell lines for chromatin immunoprecipitation (ChIP) assays
- to treat control adult and embryonic thymi cells during staining
- to remove RNA from affibody–mRNA fusion clones before clone screening
Biochem/physiol Actions
RNaseA is an endoribonuclease that catalyzes the degradation of phosphodiester linkages with a pyrimidine base at the 3′ -position on single-stranded RNA via, transphosphorylation and hydrolysis mechanism.
Warning
Toxicity: Standard Handling (A)
Legal Information
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
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Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
10 - Combustible liquids
wgk_germany
WGK 2
Certificates of Analysis (COA)
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