550701-M

Pyruvate Oxidase, Aerococcus viridans

Native pyruvate oxidase from Aerococcus viridans. Catalyzes the oxidation of pyruvate to acetylphosphate, carbon dioxide, and hydrogen peroxide in the presence of phosphate.

• Synonym(s): Pyruvate Oxidase, Aerococcus viridans
• CAS Number: 9001-96-1
• Enzyme Commission number: 1.2.3.3 (BRENDA, IUBMB)
• MDL number: MFCD00132148
• UNSPSC Code: 12352202
• NACRES: NA.54

Properties

• Quality Level: 100
• form: lyophilized solid
• specific activity: ≥25 U/mg
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; desiccated (hygroscopic); protect from light
• impurities: ≤0.006% GPT
• color: yellow
• pI: 4.0
• solubility: aqueous buffer: soluble
• foreign activity: GOT and lactate oxidase ≤0.002%; catalase ≤0.3%
• storage temp.: −20°C

Description

General description

Native pyruvate oxidase from Aerococcus viridans. Catalyzes the oxidation of pyruvate to acetylphosphate, carbon dioxide, and hydrogen peroxide in the presence of phosphate.

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit is defined as the amount of enzyme which generates 1 µmol of H₂O₂ per min at 37°C, pH 6.5.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Hager, L.P. and Lipmann, F. 1955. Methods Enzymol. 1, 482.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• pictograms: GHS08
• signalword: Danger
• hcodes: H334
• pcodes: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable