539562-M

PKC_ε Peptide Substrate

Pseudosubstrate region of the ε-isotype of protein kinase C (PKCε), with an alanine to serine substitution.

• Synonym(s): PKC_ε Peptide Substrate, Peptide-ε, ERMRPRKRQGSVRRRV, Protein Kinase C _ε Peptide Substrate, Peptide-ε, ERMRPRKRQGSVRRRV, Protein Kinase C_ε Peptide Substrate
• Empirical Formula (Hill Notation): C₈₃H₁₅₅N₃₉O₂₁S
• Molecular Weight: 2067.43
• UNSPSC Code: 12352200

Properties

• Quality Level: 100
• assay: ≥95% (HPLC)
• form: solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; desiccated (hygroscopic)
• color: white
• solubility: water: 10 mg/mL
• storage temp.: −20°C

Description

General description

Pseudosubstrate region of the ε-isotype of protein kinase C (PKC_ε) with an alanine to serine substitution. PKC_ε exhibits an apparent specificity for this peptide (K_m = 68 µM).

Biochem/physiol Actions

Km = 68 µM for PKCε

Primary Target
Pseudosubstrate region of the ε-isotype of protein kinase C (PKCε)

Warning

Toxicity: Standard Handling (A)

Sequence

H-Glu-Arg-Met-Arg-Pro-Arg-Lys-Arg-Gln-Gly-Ser-Val-Arg-Arg-Arg-Val-OH

Physical form

Supplied as a trifluoroacetate salt.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Schaap, D. and Parker, P.J. 1990. J. Biol. Chem. 265, 7301.
Schaap, D., et al. 1989. FEBS Lett. 243, 351.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable