528208

Plasminogen Activator Inhibitor-1, Mutant, Human, Recombinant

The Plasminogen Activator Inhibitor-1, Mutant, Human, Recombinant controls the biological activity of Plasminogen Activator. This small molecule/inhibitor is primarily used for Protease Inhibitors applications.

• Synonym(s): PAI-1, Mutant
• UNSPSC Code: 12352200

Properties

• Quality Level: 100
• assay: ≥95% (SDS-PAGE)
• form: liquid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; avoid repeated freeze/thaw cycles
• shipped in: wet ice
• storage temp.: −70°C

Description

General description

Highly purified preparation of an altered form of human PAI-1 containing four mutated amino acids. Mutant PAI-1 is virtually unable to go latent and is stable at elevated temperature and pH for extended periods of time (t_½ = 145 h at 37°C, pH 7.4). Inhibits uPA (K_i = 5.1 x 10⁶ M^-1sec^-1) and tPA (K_i = 7.9 x 10⁵ M^-1sec^-1).

Highly purified preparation of an altered form of human PAI-1 containing four mutated amino acids. Mutant PAI-1 is virtually unable to go latent and is stable at elevated temperature and pH for extended periods of time (t_½ = 145 hours at 37°C, pH 7.4). Inhibits uPA (K_i = 5.1 x 10⁶ M^-1 sec^-1) and tPA (K_i = 7.9 x 10⁵ M^-1 sec^-1).

Biochem/physiol Actions

Cell permeable: no

Primary Target
Plasminogen Activator

Product does not compete with ATP.

Reversible: no

Target K_i: 5.1 x 106 M-1 sec-1, 7.9 x 105 M-1 sec-1 against uPA and tPA, respectively

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Physical form

In 100 mM NaCl, 50 mM sodium phosphate buffer, 1 mM EDTA, pH 6.6.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Other Notes

Berkenpas, M.B., et al. 1995. EMBO J. 14, 2969.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable