495320

ODQ

A cell-permeable, potent and selective inhibitor of nitric oxide (NO)-sensitive guanylyl cyclase (IC₅₀ = 20 nM).

• Synonym(s): ODQ, 1H-[1,2,4]Oxadiazolo[4,3-a]quinoxalin-1-one
• Empirical Formula (Hill Notation): C₉H₅N₃O₂
• CAS Number: 41443-28-1
• Molecular Weight: 187.15
• MDL number: MFCD00792620
• UNSPSC Code: 12352200
• NACRES: NA.77

Properties

• Quality Level: 100
• assay: ≥98% (TLC and HPLC)
• form: powder
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; protect from light
• color: light yellow
• solubility: DMSO: 25 mg/mL
• shipped in: ambient
• storage temp.: −20°C
• InChI: 1S/C9H5N3O2/c13-9-12-7-4-2-1-3-6(7)10-5-8(12)11-14-9/h1-5H
• InChI key: LZMHWZHOZLVYDL-UHFFFAOYSA-N

Description

General description

A cell-permeable, potent and selective inhibitor of nitric oxide (NO)-sensitive guanylyl cyclase (IC₅₀ = 20 nM). In incubated cerebellum slices, ODQ reversibly inhibits the NO-dependent cGMP response to glutamate receptor agonists without affecting NOS activity. It does not chemically inactivate NO; however, it does inhibit cGMP generation in response to NO donors. ODQ does not inhibit NO-mediated macrophage toxicity, a phenomenon unrelated to cGMP, nor does it affect the activity of particulate guanylyl cyclase or adenylyl cyclase.

A potent and selective inhibitor of nitric oxide (NO)-sensitive guanylyl cyclase (IC₅₀ = 20 nM). In incubated cerebellum slices, ODQ reversibly inhibits the NO-dependent cGMP response to glutamate receptor agonists without affecting NOS activity. It does not chemically inactivate NO; however, it does inhibit cGMP generation in response to NO donors. ODQ fails to inhibit NO-mediated macrophage toxicity, a phenomenon unrelated to cGMP, nor does it affect the activity of particulate guanylyl cyclase or adenylyl cyclase.

Biochem/physiol Actions

Cell permeable: yes

Primary Target
(NO)-sensitive guanylyl cyclase

Product does not compete with ATP.

Reversible: yes

Target IC₅₀: 20 nM against nitric oxide (NO)-sensitive guanylyl cyclase

Warning

Toxicity: Irritant (B)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Other Notes

Wang, D., et al. 1998. J. Biol. Chem. 273, 33027.
Southam, E., et al. 1996. Br. J. Pharmacol. 119, 527.
Brunner, F., et al. 1995. FEBS Lett.376, 262.
Garthwaite, J., et al. 1995. Mol. Pharmacol.48, 184.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable