264151

Caspase-3 Substrate VII, Fluorogenic

Fluorogenic caspase-3 substrate.

• Synonym(s): Caspase-3 Substrate VII, Fluorogenic, Ac-DEVD-AFC
• Empirical Formula (Hill Notation): C₃₀H₃₄F₃N₅O₁₃
• Molecular Weight: 729.61
• UNSPSC Code: 12352200
• NACRES: NA.77

Properties

• Quality Level: 100
• assay: ≥95% (HPLC)
• form: lyophilized solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; desiccated; protect from light
• color: white
• solubility: DMSO: 5 mg/mL
• fluorescence: λ_ex ~400 nm; λ_em ~505 nm
• shipped in: ambient
• storage temp.: −20°C

Description

General description

Fluorogenic caspase-3 substrate (excitation maximum: ~400 nm; emission maximum: ~505 nm). Also acts as a substrate for granzyme B-processed ICE-LAP3, caspase-6, caspase-7, caspase-8, and caspase-10.

Fluorogenic caspase-3 substrate. Also acts as a substrate for granzyme B-processed caspase-6, caspase-7, caspase-8, and caspase-10.

Biochem/physiol Actions

Cell permeable: no

Primary Target
Fluorogenic caspase-3 substrate

Product does not compete with ATP.

Reversible: no

Warning

Toxicity: Standard Handling (A)

Sequence

Ac-Asp-Glu-Val-Asp-AFC

Reconstitution

Following reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Keramaris, E., et al. 2000. Mol. Cell Neurosci. 15, 368.
Thornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable