228180

Cholesterol Esterase, Microbial

Cholesterol Esterase, Microbial, CAS 9026-00-0, is a native enzyme that catalyzes hydrolysis and formation of cholesterol esters, ranging from cholesterol acetate to cholesterol stearate.

• Synonym(s): Cholesterol Esterase, Microbial, CE, Sterolester Acylhydrolase
• CAS Number: 9026-00-0
• Enzyme Commission number: 3.1.1.13 (BRENDA, IUBMB)
• MDL number: MFCD00071052
• UNSPSC Code: 12352202
• NACRES: NA.54

Properties

• Quality Level: 100
• form: powder
• specific activity: ≥100 units/mg protein; ≥70 units/mg dry wt.
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; desiccated; protect from light
• color: light brown
• solubility: 10 mM potassium phosphate/sodium hydroxide, 0.1% BSA, pH 7.5: soluble
• foreign activity: Catalase ≤1.5%
• shipped in: ambient
• storage temp.: −20°C

Description

General description

Native, microbial cholesterol esterase. Catalyzes hydrolysis and formation of cholesterol esters, ranging from cholesterol acetate to cholesterol stearate.

Packaging

Packaged under inert gas

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol cholesterol ester per min at 37°C, pH 6.5.

Reconstitution

Reconstitute just prior to use. Enzyme will lose activity in solution upon storage.

Other Notes

Allain, C.C., et al. 1974. Clin. Chem.20, 470.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• pictograms: GHS08
• signalword: Danger
• hcodes: H334
• pcodes: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable