176872
Angiotensin Converting Enzyme-2, His•Tag®, Human, Recombinant, NSO Cells
Synonym(s):
ACE Homolog, ACEH, ACE-2
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About This Item
UNSPSC Code:
12352202
NACRES:
NA.32
Recommended Products
recombinant
expressed in NSO cells
Quality Level
assay
≥90% (SDS-PAGE)
form
liquid
specific activity
≥0.8 units/μg protein
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
impurities
≤1.0 EU/μg Endotoxins (EU/μg ACE-2)
shipped in
wet ice
storage temp.
−70°C
General description
Recombinant, human ACE-2 ectodomain (amino acids 1-740) fused at the C-terminus to a His•Tag sequence and expressed in NSO cells. ACE-2 cleaves angiotensins I and II as a carboxypeptidase and plays an important role in regulating cardiovascular and renal functions. Native ACE-2 is an integral membrane zinc metalloprotease whose activity is inhibited by EDTA. By SDS-PAGE, the apparent molecular mass of the glycosylated protein is ~120 kDa under either reducing or non-reducing conditions.
Recombinant, human ACE-2 ectodomain (amino acids 1-740) fused at the C-terminus to a His•Tag sequence and expressed in NSO cells. Native ACE-2 is an integral membrane zinc metalloprotease that can be inhibited by EDTA. ACE-2 cleaves angiotensins I and II. Plays an important role in regulating cardiovascular and renal functions. By SDS-PAGE, the apparent molecular mass of the glycosylated protein is ~120 kDa under either reducing or non-reducing condition.
Packaging
Please refer to vial label for lot-specific concentration.
Warning
Toxicity: Standard Handling (A)
Unit Definition
One unit is defined as the amount of enzyme that will cleave 1 nmol MCA-YVADAPK(DNP)-OH per min at 25°C, pH 7.5.
Reconstitution
Following initial thaw, aliquot and freeze (-70°C).
Other Notes
Tikellis, C., et al. 2003. Hypertension41, 390.
Tipnis, S.R., et al. 2000. J. Biol. Chem.275, 33238.
Tipnis, S.R., et al. 2000. J. Biol. Chem.275, 33238.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class
10 - Combustible liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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