1.50245

Purospher^® STAR NH2 (5 µm) HPLC Columns

L × I.D. 250 mm x 4 mm LiChroCART^™ HPLC cartridge, manuCART^™ holder required for use

• Synonym(s): Purospher^® STAR NH2 (5 µm) LiChroCART^™ 250-4
• UNSPSC Code: 41115709
• NACRES: SB.52

Properties

• product name: Purospher^® STAR NH2 (5 µm), L × I.D. 250 mm x 4 mm LiChroCART^™ HPLC cartridge, manuCART^™ holder required for use
• material: stainless steel column
• Quality Level: 100
• agency: suitable for USP L8
• product line: Purospher^®
• feature: endcapped: no
• packaging: 1 ea of
• extent of labeling: 3.5% Carbon loading
• parameter: 250 bar pressure; 60 °C max. temp.
• technique(s): HPLC: suitable; LC/MS: suitable; mass spectrometry (MS): suitable (Low - Medium)
• column L × I.D.: 25 cm × 4 mm
• surface area: 330 m²/g
• matrix: fully porous particle
• matrix active group: NH₂ bonding phase
• particle size: 5 μm
• pore size: 120 Å pore size
• operating pH range: 2-7.5
• separation technique: hydrophilic interaction (HILIC); normal phase; reversed phase
• storage temp.: no temp limit

Description

General description

Purospher^® STAR NH2, 5 µm HPLC columns are widely used for carbohydrate analysis, with a typical mobile phaseconsisting of acetonitrile and water. In terms of their polarity, these columns lie between bare silica and reversed-phase silica. Hence, Purospher^® STAR NH2 can also be used as an ion-exchanger. In acidic solutions, the NH2-groups are protonated (-NH3 +X-) and therefore display the characteristics of a weak anion exchanger. Medium polarity Purospher^® STAR NH2 columns possess hydrophilic as well as hydrophobic properties, thus can be used under both reversed-phase (HILIC) and normal-phase conditions. However, retention is weaker than on silica or RP-supports. Purospher^® STAR NH2 columns are based on ultra-pure fully porous silica particles (Type B). Purospher^® STAR NH2, 5 µm LiChroCART^™ 250-4 is a cartridge column which requires a manuCART^™ cartridge column holder part number 1.51486.0001.The recommended guard column is Purospher^® STAR NH2 (5 µm) LiChroCART^™ 4-4 part number 1.50267.0001. LiChroCART^™ cartridge columns mounted with a manuCART^™ cartridge column holder is hand-tight only and have therefore a maximum pressure stability of 250 bar.The column dimension of 250 mm length x 4 mm I.D. is the optimal choice for the HPLC separation of complex.Learn more about Purospher^® STAR HPLC and UHPLC ColumnsLearn more about the Supelco HPLC Column portfolio and Method development:Brochure: HPLC and UHPLC Column Selection GuideBrochure: A Practical Guide to HPLC - Method Development Guide

Application

Intended to be used in the separation of alcohols,carboxylic acids, fat soluble vitamins, nucleotides, steroids, sugars, sugar alcohols, sweeteners, and water soluble vitamins.

Features and Benefits

• Ultra-pure silica (99.999%)
• Low silanol activity
• Reproducible results from run-to-run and batch-to-batch

Analysis Note

Pressure: ≤ 60 bar
Theoretical Plates (N/m) (2-Nitrotoluene): ≥ 50000
Symmetry (2-Nitrotoluene): 0.8 - 1.4
Capacity factor (2-Nitrotoluene): 0.5 – 1.8

Legal Information

LICHROCART is a trademark of Merck KGaA, Darmstadt, Germany

MANUCART is a trademark of Merck KGaA, Darmstadt, Germany

Purospher is a registered trademark of Merck KGaA, Darmstadt, Germany