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Key Documents

14-865

Sigma-Aldrich

p53-GST (recombinant protein)

Synonym(s):

transformation related protein 53

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About This Item

UNSPSC Code:
12352200
eCl@ss:
32160405
NACRES:
NA.41

biological source

human

Quality Level

assay

~95% (SDS-PAGE)

mol wt

Mw 72 kDa

manufacturer/tradename

Upstate®

concentration

0.5 mg/mL

technique(s)

activity assay: suitable

NCBI accession no.

UniProt accession no.

shipped in

dry ice

General description

N-terminal GST-tagged, recombinant full length human P53 protein, expressed in E. coli. Purified using glutathione agarose chromatography.
Product Source: Full-length human p53 protein, expressed in e. coli.

Quality

Routinely evaluated by SDS-PAGE and Coomassie Stain

Physical form

Glutathione agarose chromatography
Recombinant protein in buffer containing 20 mM Tris-HCl, pH 8.0, 100 mM KCl, 5mM glutathione, 0.5 mM EDTA, 0.5 mM DTT, 1 mM PMSF containing 20% glycerol.

Storage and Stability

1 year at -20°C

Other Notes

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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María Pedrero et al.
Biosensors, 6(4) (2016-11-24)
An amperometric magnetoimmunosensor for the determination of human p53 protein is described in this work using a sandwich configuration involving the covalent immobilization of a specific capture antibody onto activated carboxylic-modified magnetic beads (HOOC-MBs) and incubation of the modified MBs

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