14-601-M
ROKβ/ROCK-I Protein, active, 10 µg
Active, N-terminal His6-tagged, recombinant, human ROCK-I amino acids 17-535, autoactivated by incubating with Mg/ATP & redialysed to remove excess ATP. For use in Kinase Assays.
Synonym(s):
Rho-associated protein kinase, p160ROCK
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About This Item
UNSPSC Code:
12352202
eCl@ss:
32160405
NACRES:
NA.41
Recommended Products
biological source
human
Quality Level
recombinant
expressed in baculovirus infected Sf21 cells
form
liquid
shelf life
6 mo.
mol wt
Mw 61.4 kDa
manufacturer/tradename
Upstate®
technique(s)
activity assay: suitable (kinase)
NCBI accession no.
UniProt accession no.
storage temp.
−70°C
Gene Information
human ... ROCK1(6093)
General description
Research area: Apoptosis
N-terminal His6-tagged, recombinant, human ROCK-I amino acids 17-535; autoactivated by incubating with Mg/ATP and redialysed to remove excess ATP
Product Source: expressed by baculovirus in Sf21 insect cells
N-terminal His6-tagged, recombinant, human ROCK-I amino acids 17-535; autoactivated by incubating with Mg/ATP and redialysed to remove excess ATP
Product Source: expressed by baculovirus in Sf21 insect cells
Application
Research Category
Inflammation & Immunology
Inflammation & Immunology
Biochem/physiol Actions
G protein, RhoA/Rho kinase 1 (ROCK1) is a serine/threonine kinase. It plays a vital role in the regulation of cell growth, and invasive and metastatic progression of various types of human cancers. ROCK1 is involved in the regulation of apoptosis or programmed cell death in various cell types. Overexpression of ROCK1 has been observed in various tumors, including glioblastoma, melanoma, osteosarcoma, and hepatocellular carcinoma. This protein is known to enhance actin-myosin contractility by phosphorylating regulatory myosin light chains (MLCs). It also plays a role in cell motility through the modulation of integrin β1-activated focal adhesion kinase (FAK) signaling.
Packaging
Also available in 250 µg size --call for pricing and availability and reference catalog number 14-601M when ordering the 250 µg size.
Quality
routinely evaluated by phosphorylation of Long S6 substrate peptide (Catalog #12-420)
Physical form
Ni2+/NTA-agarose
Storage and Stability
6 months at -70°C
Other Notes
For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
signalword
Warning
hcodes
Hazard Classifications
Skin Sens. 1
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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