14-276-M
Akt1/PKBα Protein, active, 15 µg
Active, recombinant full-length human Akt1, containing N-terminal His6 tag, for use in IP-Kinase Assays & Kinase Assays.
Synonym(s):
Protein kinase B alpha, Proto-oncogene c-Akt, RAC, RAC-PK-alpha
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About This Item
UNSPSC Code:
12352202
eCl@ss:
32160405
NACRES:
NA.26
Recommended Products
biological source
human
Quality Level
recombinant
expressed in baculovirus infected Sf21 cells
form
liquid
shelf life
1 yr
mol wt
Mw 59.9 kDa
manufacturer/tradename
Upstate®
technique(s)
activity assay: suitable (IP-kinase)
activity assay: suitable (kinase)
solubility
soluble
NCBI accession no.
UniProt accession no.
storage temp.
−70°C
Gene Information
human ... AKT1(207)
General description
Research area: CELL SIGNALING
Product Source: Expressed by baculovirus in Sf21 insect cells
Recombinant full-length human Akt1, containing N-terminal His6-tag.
Product Source: Expressed by baculovirus in Sf21 insect cells
Recombinant full-length human Akt1, containing N-terminal His6-tag.
Biochem/physiol Actions
Protein Target: Akt1/PKBα
Target Sub-Family: AGC
Akt, also known as protein Kinase B (PKB), is a crucial protein involved in various cell signaling pathways. It modifies and controls the activities of numerous proteins involved in a wide range of cellular and physiological processes. Some of these processes include cell survival, apoptosis, cell proliferation, transcription, cell migration, and metabolism. Akt also helps in influencing angiogenesis, and normal development of embryos. Akt regulates cell survival through the phosphorylation of mitogen-activated protein kinase kinase kinase 5 (MAP3K5), which is involved in the cellular apoptotic response. The Akt occupies a pivotal position in the phosphoinositide 3-kinase-protein kinase B-mammalian target of rapamycin (PI3K-PKB-mTOR) signaling pathway. Genetic mutations occurring upstream of Akt in this cascade can result in uncontrolled cell proliferation and the development of cancer. In addition abberent Akt activation is associated with the development of type-2 diabetes.
Target Sub-Family: AGC
Akt, also known as protein Kinase B (PKB), is a crucial protein involved in various cell signaling pathways. It modifies and controls the activities of numerous proteins involved in a wide range of cellular and physiological processes. Some of these processes include cell survival, apoptosis, cell proliferation, transcription, cell migration, and metabolism. Akt also helps in influencing angiogenesis, and normal development of embryos. Akt regulates cell survival through the phosphorylation of mitogen-activated protein kinase kinase kinase 5 (MAP3K5), which is involved in the cellular apoptotic response. The Akt occupies a pivotal position in the phosphoinositide 3-kinase-protein kinase B-mammalian target of rapamycin (PI3K-PKB-mTOR) signaling pathway. Genetic mutations occurring upstream of Akt in this cascade can result in uncontrolled cell proliferation and the development of cancer. In addition abberent Akt activation is associated with the development of type-2 diabetes.
Packaging
Also available in 250μg size, please inquire for pricing and availability.
Quality
Routinely evaluated by phosphorylation of Crosstide. Please refer to the Certificate of Analysis for more information.
Physical form
Ni2+/NTA and glutathione agarose
Supplied in 50mM Tris/HCl pH7.5, 150mM NaCl, 150mM imidazole, 1mM benzamidine, 0.2mM PMSF, 0.1mM EGTA, 0.07% 2-mercaptoethanol, 0.02% Brij 35, 270mM sucrose. Frozen solution.
Storage and Stability
1 year at -70°C
Other Notes
For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
signalword
Danger
Hazard Classifications
Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2 - Skin Sens. 1
Storage Class
6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
wgk_germany
WGK 1
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Find documentation for the products that you have recently purchased in the Document Library.
A common phosphate binding site explains the unique substrate specificity of GSK3 and its inactivation by phosphorylation
Frame, S., et al
Molecular Cell, 7, 1321-1327 (2001)
Stoichiometry of site-specific protein phosphorylation estimated with phosphopeptide-specific antibodies.
Miinea, Cristinel P and Lienhard, Gustav E
Biotechniques, 34, 828-831 (2003)
B Bauer et al.
The Journal of biological chemistry, 276(34), 31627-31634 (2001-06-19)
Protein kinase C theta (PKC theta) is known to induce NF-kappa B, an essential transcriptional element in T cell receptor/CD28-mediated interleukin-2 production but also T cell survival. Here we provide evidence that PKC theta is physically and functionally coupled to
Madhavi J Rane et al.
The Journal of biological chemistry, 278(30), 27828-27835 (2003-05-13)
Activation of the serine-threonine kinase Akt by cytokines, chemokines, and bacterial products delays constitutive neutrophil apoptosis, resulting in a prolonged inflammatory response. We showed previously that Akt exists in a signaling complex with p38 MAPK, MAPK-activated protein kinase-2 (MAPKAPK-2), and
P Cohen et al.
FEBS letters, 410(1), 3-10 (1997-06-23)
The initial steps in insulin signal transduction occur at the plasma membrane and lead to the activation of phosphatidylinositide (PtdIns) 3-kinase and the formation of PtdIns(3,4,5,)P3 in the inner leaflet of the plasma membrane which is then converted to PtdIns(3,4)P2
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