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Key Documents

1.37159

SAFC

DL-Dithiothreitol

EMPROVE® ESSENTIAL

Pharma Manufacturing

Synonym(s):

DL-Dithiothreitol, Cleland’s reagent, DTT, threo-1,4-Dimercapto-2,3-butanediol, threo-1,4-Dimercapto-2,3-butanediol, Cleland’s reagent

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About This Item

Linear Formula:
HSCH2CH(OH)CH(OH)CH2SH
CAS Number:
Molecular Weight:
154.25
Beilstein/REAXYS Number:
1719757
MDL number:
EC Index Number:
222-468-7

biological source

synthetic

Quality Level

product line

EMPROVE® ESSENTIAL

mp

41-44 °C (lit.)

application(s)

pharma/biopharma processes

storage temp.

−20°C (−15°C to −25°C)

SMILES string

O[C@H](CS)[C@H](O)CS

InChI

1S/C4H10O2S2/c5-3(1-7)4(6)2-8/h3-8H,1-2H2/t3-,4-/m1/s1

InChI key

VHJLVAABSRFDPM-QWWZWVQMSA-N

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General description

Our SAFC® portfolio of high-quality products for biopharmaceutical processing withstands strict quality control procedures and is produced according to MQ-500 requirements as defined by the M-Clarity program.
M-Clarity Program
As part of our Emprove® Program, our raw materials are offered with Emprove® Dossiers which provide comprehensive, up-to-date documentation to help you navigate regulatory challenges, manage risks, and improve your manufacturing processes.
Our comprehensive portfolio of downstream process chemicals not only provides biopharmaceutical manufacturers with high-quality raw materials for production of classical and novel therapies, but also helps them get to market faster and simplify regulatory challenges. Ranging from non-GMP grades for low-risk application, to IPEC-PQG GMP for higher-risk applications, we have products covering all your manufacturing needs.

Application

DTT is an excellent reagent chemical used to reduce disulfide bonds commonly found in bioprocessing. DTT is used in buffer formulations to maintain and stabilize free -SH in applications such as protein based biotherapeutics and enzyme stability.
An excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol. Typically, a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).

Packaging

1.37159.0010 / 10 g / Amber glass bottle
1.37159.0100 / 100 g / Amber glass bottle
1.37159.1000 / 1 kg / LDPE bag with outer HDPE drum
1.37159.9010 / 10 kg / LDPE bag with outer HDPE drum

Legal Information

Emprove is a registered trademark of Merck KGaA, Darmstadt, Germany
SAFC Pharma is a registered trademark of Sigma-Aldrich Co. LLC

pictograms

CorrosionExclamation mark

signalword

Danger

Hazard Classifications

Acute Tox. 4 Oral - Eye Dam. 1 - Skin Irrit. 2

Storage Class

11 - Combustible Solids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

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