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biological source
human
Quality Level
form
liquid
manufacturer/tradename
Upstate®
concentration
2.0 mg/mL
technique(s)
western blot: suitable
shipped in
dry ice
General description
Nuclear extract prepared from human HeLa cells using a modified protocol of Dignam, et al., which contains a variety of DNA binding proteins and transcription factors.
Cell Line Description
HeLa
Quality
Routinely evaluated as a positive control for many of Upstates antibodies.
Physical form
RIPA sample buffer containing inhibitors. Frozen solution.
Storage and Stability
Stable for 6 months at -70°C from date of shipment.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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J D Dignam et al.
Nucleic acids research, 11(5), 1475-1489 (1983-03-11)
We have developed a procedure for preparing extracts from nuclei of human tissue culture cells that directs accurate transcription initiation in vitro from class II promoters. Conditions of extraction and assay have been optimized for maximum activity using the major
Tabetha Sundin et al.
Biochimie, 94(12), 2639-2648 (2012-08-21)
Isoprenoids are recognized for their ability to suppress carcinogenic processes in vivo and in vitro. We previously established that the isoprenoid, perillyl alcohol, acted mechanistically on translation of specific proteins through modulation of mechanistic target of rapamycin (mTOR) signaling. Telomerase-the
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