1.11615

Ethidium bromide

for biochemistry

• Synonym(s): Ethidium bromide, 5-Ethyl-3,8-diamino-6-phenylphenanthridinium bromide, Homidium bromide, EtBr
• CAS Number: 1239-45-8
• Molecular Weight: 394.31
• MDL number: MFCD00011724
• UNSPSC Code: 41105322
• EC Index Number: 214-984-6

Properties

• Quality Level: 100
• form: solid
• potency: 1503 mg/kg LD₅₀, oral (Rat)
• pH: 4.4 (20 °C, 10 g/L in H₂O)
• mp: 261-264 °C
• transition temp: flash point >100 °C
• solubility: 40 g/L
• density: 1.01 g/cm³ at 20 °C
• bulk density: 340 kg/m³
• storage temp.: 2-30°C
• InChI: 1S/C21H19N3.BrH/c1-2-24-20-13-16(23)9-11-18(20)17-10-8-15(22)12-19(17)21(24)14-6-4-3-5-7-14;/h3-13,23H,2,22H2,1H3;1H
• InChI key: ZMMJGEGLRURXTF-UHFFFAOYSA-N

Description

General description

for biochemistry

Application

Ethidium bromide for biochemistry. CAS 1239-45-8, pH 4.4 (10 g/l, H₂O, 20 °C).

Analysis Note

Assay (perchloric acid titration, calculated on dried substance)≥ 98 %Identity (UV/VIS-Spectrum)passes testAbsorption maximum λ_max. (methanol)524 - 527 nmSpec. Absorptivity A 1%/1cm (λ_max; 0.05 g/l; methanol; calc. on dried substance)155 - 170TLC-Testpasses testLoss on drying (130 °C, 4 h)≤ 8 %

Safety Information

• pictograms: GHS06,GHS08
• signalword: Danger
• hcodes: H302 - H330 - H341
• pcodes: P202 - P260 - P264 - P271 - P301 + P312 - P304 + P340 + P310
• Hazard Classifications: Acute Tox. 1 Inhalation - Acute Tox. 4 Oral - Muta. 2
• Storage Class: 6.1A - Combustible, acute toxic Cat. 1 and 2 / very toxic hazardous materials
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable