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MilliporeSigma
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Key Documents

1.10285

Millipore

LB Broth

GranuCult® plus, granular, pack of 500 g or 5 kg, for Escherichia coli

Synonym(s):

E. coli culture medium, GranuCult® plus LB broth (MILLER), Luria Bertani Broth, Luria Bertani Medium

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About This Item

UNSPSC Code:
41106213
NACRES:
NA.74

sterility

non-sterile

Quality Level

200

form

granular

manufacturer/tradename

GranuCult® plus

packaging

pack of 500 g or 5 kg

storage condition

dry media (Keep tightly closed)

technique(s)

microbiological culture: suitable

color

light yellow to brown

pH

6.8-7.2 (25 °C in H2O, after autoclaving)

application(s)

microbiology
pharmaceutical

storage temp.

15-25°C

suitability

Escherichia coli

General description

GranuCult® plus LB broth (MILLER) formulation supports the growth of Escherichia coli. Casein peptone and yeast extracts supply essential growth factors, such as nitrogen, carbon, sulfur, minerals, and vitamins.

Application

GranuCult® plus LB broth (MILLER) is suitable for the cultivation of E. coli in fermentation and molecular genetic studies.

Features and Benefits

  • GranuCult® offers superior granulated culture media
  • Safe and sustainable due to reduced risks associated with fine dust and toxic substance inhalation, resulting in a safer work environment
  • Excellent wettability, solubility, and free-flowing properties
  • Convenient, with minimal component separation and clumping, even under warm or humid conditions
  • High batch-to-batch reproducibility
  • Prolonged shelf life of up to five years
  • High number of test strains exceeding all regulatory demands
  • Granulation technology allows many supplements to be included, with no need to add these separately

Linkage

Replaces: 1.10285.5007

Analysis Note

Appearance (clearness): clear
Appearance (color): yellowish to yellowish brown
pH-value (25 °C): 6.8 - 7.2
Inoculum on reference medium (Escherichia coli ATCC 25922 (WDCM 00013)): ≤ 100
Inoculum on reference medium (Escherichia coli ATCC 11775 (WDCM 00090)): ≤ 100
Inoculum on reference medium (Escherichia coli DSM 6897 (DH5alpha)): ≤ 100
Inoculum on reference medium (Escherichia coli ATCC 47092 (BL21)): ≤ 100
Growth (Escherichia coli ATCC 25922 (WDCM 00013)): good to very good
Growth (Escherichia coli ATCC 11775 (WDCM 00090)): good to very good
Growth (Escherichia coli DSM 6897 (DH5alpha)): good to very good
Growth (Escherichia coli ATCC 47092 (BL21)): good to very good
Incubation: 24 ± 2 hours at 37 ± 1 °C, aerobic

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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