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1.09215

Sigma-Aldrich

Ziehl-Neelsen carbol-fuchsin solution

for microscopy

Synonym(s):

Carbol fuchsin solution

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About This Item

UNSPSC Code:
41116130
NACRES:
NA.74

Quality Level

400

form

liquid

IVD

for in vitro diagnostic use

technique(s)

microbe id | staining: suitable

transition temp

flash point 47 °C

density

0.99 g/cm3 at 20 °C

application(s)

clinical testing
diagnostic assay manufacturing
hematology
histology

storage temp.

15-25°C

General description

Ziehl-Neelsen carbolfuchsin solution - for microscopy, is a ready-to use solution used in the Ziehl-Neelsen hot staining for the microscopic investigation of acid-fast bacteria(AFB). The cell wall of acid-fast bacteria has a high proportion of wax and lipids and hence absorbs dyes only very slowly. The most efficient staining method is the hot staining according to Ziehl-Neelsen. In this method, carbolfuchsin solution is applied to the specimen and heated. This heating process accelerates the rate at which the fuchsin dye is absorbed and thus also that of the formation of the mycolate-fuchsin complex in the cell wall. Once the acid-fast bacteria have absorbed the fuchsin dye, it is virtually impossible to decolorize them again, even when they are intensively treated with a decolorizing solution such as e. g. hydrochloric acid in ethanol (Product number 1.00327). Accordingly, acid-fast bacteria are termed as acid- and alcohol-fast for staining, and are stained red in the microscopic visualization. Correspondingly, all non-acid-fast microorganisms are counterstained with an appropriate dye. In this instruction for use Löfföer′s methylene blue solution(Cat. no. 101287) is used for counterstaining. Those three solutions are each 500 ml and are contained in the AFB-color modified staining kit (Product number 1.00497).
The 500 ml bottle of Ziehl-Neelsen carbolfuchsin solution provides approx. 250 stainings. Pretreatment of the specimens with Sputofluol™ dissolves the bacteria from the surrounding viscid sputum and cell material. Ziehl-Neelsen carbolfuchsin solution - for microscopy is an IVD product and CE registered. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

Application

For Microscopy. Ready-to-use staining solution for the purpose of bacteriological and histological investigation of sample material of human origin.

Legal Information

SPUTOFLUOL is a trademark of Merck KGaA, Darmstadt, Germany

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Danger

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Flam. Liq. 3 - Muta. 2 - Skin Corr. 1B

Storage Class

3 - Flammable liquids

wgk_germany

WGK 3

flash_point_f

116.6 °F

flash_point_c

47 °C

Certificates of Analysis (COA)

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