1.05642
HPTLC plates, Silica gel 60 F₂₅₄
pkg of 50 plates, plate L × W 20 cm × 10 cm, glass support
Synonym(s):
HPTLC Silica Gel Plates
About This Item
Recommended Products
material
glass support
silica gel 60 matrix
Quality Level
feature
binder Organic Polymer
fluorescent indicator
packaging
pkg of 50 plates
technique(s)
thin layer chromatography (TLC): suitable
layer thickness
200 μm
plate L × W
20 cm × 10 cm
particle size
5-6 μm
pore size
60 Å medium pore diameter
storage temp.
2-30°C
Related Categories
General description
Application
Linkage
Analysis Note
Pore volume (N₂-isotherm): 0.74 - 0.84 ml/g
d 50 (laser diffraction, size distribution): 5 - 7 µm
Layer thickness: 150 - 200 µm
Deviation of layer thickness per plate: ≤ 35 µm
Chromatographic testings:
A) Colour test
hRf-values
- bleu vif organol, colour test, lipophile:
17 - 22
- Ceres Black G, colour test, lipophile: 38 - 43
- ceres Violet brn, colour test, lipophile: 58 - 64
separation number
- colourtest, lipophile:
≥ 10.0
B) Steroid test
hRf-values
- hydrocortisone, steroid test:
23 - 29
- Reichstein S, steroid test: 36 - 45
- methyltestosterone, steroid test: 43 - 52
separation number
- steroid test:
≥ 10.5
Typical value determined on a conditioned plate
Eluent A) Toluene (45% rel.humidity)
Eluent B) Ethyl acetate/Toluene (95+5 v/v; 20% rel.humidity)
Certificates of Analysis (COA)
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Protocols
HPTLC was used to analyze caffeine in coffee. Filtration of coffee through a PTFE syringe filter was the only sample preparation. Quantitation was conducted with a TLC scanner.
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Boiled coffee samples were extracted and filtered with a 0.45 μm Millex syringe filter.
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