07-690

Anti-Histone H3 Antibody, CT, pan

serum, Upstate^®

• Synonym(s): H3, Histone H3, H3 histone family, member T, histone 3, H3, histone cluster 3, H3
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: serum
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: human, mouse, yeast, rat, chicken, Saccharomyces cerevisiae
• manufacturer/tradename: Upstate^®
• technique(s): ChIP: suitable; western blot: suitable
• NCBI accession no.: NM_002107.3
• UniProt accession no.: Q16695
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: human ... H3C1(8350) ; mouse ... H3C1(360198) ; rat ... H3C1(679994)

Description

General description

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

Specificity

Broad species cross-reactivity expected due to sequence homology.

Recognizes C-Terminal region of Histone H3, Mr 17 kDa

Immunogen

KLH-conjugated, synthetic peptide corresponding to the C-terminus of human Histone H3.

Application

Chromatin Immunoprecipitation:
This antibody has been reported by an independent laboratory to immunoprecipitate chromatin from yeast.

Use Anti-Histone H3 Antibody, CT, pan (rabbit polyclonal antibody) validated in ChIP, WB to detect Histone H3 also known as H3, Histone H3, H3 histone family member T, histone 3 H3, histone cluster 3 H3.

Quality

Routinely evaluated by immunoblot on acid extracted proteins from untreated, sodium butyrate or colcemid treated HeLa cells.

Western Blot Analysis:
1:25,000-1:50,000 dilution of this lot detected Histone H3 in a modification independent manner in acid extracted proteins from untreated, sodium butyrate or colcemid treated HeLa cells.

Target description

Approx. 17 kDa

Physical form

Rabbit antiserum diluted 1:2 in storage buffer (0.02 M Phosphate, 0.25 M NaCl, 0.1% sodium azide) before the addition of glycerol to 30%.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 1