07-632

Anti-NR2A Antibody

Upstate^®, from rabbit

• Synonym(s): N-methyl D-aspartate receptor subtype 2A, N-methyl-D-aspartate receptor channe, subunit epsilon-1, N-methyl-D-aspartate receptor subunit 2A, NMDA receptor subtype 2A, glutamate receptor, ionotropic, N-methyl D-aspartate 2A
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: rat, mouse
• manufacturer/tradename: Upstate^®
• technique(s): immunoprecipitation (IP): suitable; western blot: suitable
• isotype: IgG
• NCBI accession no.: NM_008170
• UniProt accession no.: Q12879
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... GRIN2A(2903)

Description

General description

NR2 or glutamate receptor, ionotropic, N-methyl D-aspartate 2 (NMDA2; NMDAR2) along with the NR1 subunit forms a heterodimer that make up the NMDA receptor. By alternative splicing there are various isoforms of NR2 classified as NR2A through D. The NMDA receptor (NMDAR) is characterized as a ionotropic receptor for glutamate. As the name suggests, (NMDA (N-methyl D-aspartate) is an agonist for the receptor. A distinct characteristic of the NMDA receptor is that it is both ligand-gated and voltage-dependent based on its sensitivity to magnesium. The NR1 subunit binds glycine while the NR2 subunit binds glutamine for activation. Activated NMDA receptors result in the opening of an ion channel that is nonselective to cations. The result is the flow of Na+ and small amounts of Ca2+ ions into the cell and K+ out of the cell. Calcium flux by the NMDARs has been implicated in synaptic plasticity, a mechanism in the cell for learning and memory.

Specificity

Recognizes NR2A, MW ~170 kDa.

Immunogen

6His-tagged fusion protein corresponding to amino acids 1265-1464 of mouse NMDA receptor 2A (NR2A).

Application

Detect NR2A using this Anti-NR2A Antibody validated for use in IP & WB.

Immunoprecipitation:
4 µg of a previous lot immuno-precipitated NR2A from rat brain hippocampus membranes and rat brain microsomal preparations.

Research Category
Neuroscience

Research Sub Category
Neurotransmitters & Receptors

Quality

Evaluated by western blot in rat brain microsomal preparations (Catalog # 12-144).

Western Blot Analysis:
0.5-2 µg/mL of this antibody detected NR2A in rat brain microsomal preparations (Catalog # 12-144).

Target description

~170 kDa

Physical form

Format: Purified

Protein A purified

Purified rabbit polyclonal IgG in buffer containing 70% storage buffer 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide and 30% glycerol.

Storage and Stability

Stable for 1 year at -20ºC from date of receipt.

Analysis Note

Control
Brain tissue.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable