06-1340

Anti-phospho-IKK-epsilon (Ser172) Antibody

from rabbit, purified by affinity chromatography

• Synonym(s): Inhibitor of nuclear factor kappa-B kinase subunit epsilon, I-kappa-B kinase epsilon, IKK-E, IKK-epsilon, IkBKE, Inducible I kappa-B kinase, IKK-i
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: human, mouse
• species reactivity (predicted by homology): rat (based on 100% sequence homology)
• technique(s): western blot: suitable
• NCBI accession no.: NP_001180251
• UniProt accession no.: Q14164
• shipped in: wet ice
• target post-translational modification: phosphorylation (pSer172)
• Gene Information: human ... IKBKE(9641)

Description

General description

Inhibitor of nuclear factor kappa-B kinase subunit epsilon (UniProt: Q14164; also known as EC: 2.7.11.10, I-kappa-B kinase epsilon, IKK-E, IKK-epsilon, IkBKE, Inducible I kappa-B kinase, IKK-i) is encoded by the IKBKE (also known as IKKE, IKKI, KIAA0151) gene (Gene ID: 9641) in human. IKK-epsilon is a homodimeric serine/threonine kinase that is highly expressed in spleen, thymus, peripheral blood leukocytes, pancreas, and placenta. Its activity is induced by lipopolysaccharide and TNF-alpha. It plays an essential role in regulating inflammatory responses to viral infection, through the activation of the type I IFN, NF-kappa-B, and STAT signaling. IKK-epsilon can be either be auto-phosphorylated or phosphorylated by IKBKB/IKKB and phosphorylation at serine172 is enhanced by the interaction with ATP-dependent RNA helicase DDX3X. Its ATP-binding site resides in amino acids 15 to 23. IKK-epsilon is reported to phosphorylate inhibitors of NF-kappa-B leading to their ubiquitination and degradation. Following activation of viral RNA sensors, such as RIG-I-like receptors, it associates with DDX3X and phosphorylates interferon regulatory factors (IRFs), IRF3 and IRF7, as well as DDX3X. This activity allows subsequent homodimerization and nuclear translocation of the IRF3 leading to transcriptional activation of pro-inflammatory and antiviral genes including IFNB. In order to establish such an antiviral state, IKBKE forms several different complexes whose composition depends on the type of cell and cellular stimuli. Two isoforms of IKK-epsilon have been described that are produced by alternative splicing.

Specificity

This antibody recognizes IKK-epsilon phosphorylated at Ser172.

Immunogen

Epitope: Phosphorylated Ser172

Linear peptide corresponding to human IKKepsilon phosphorylated at Ser172.

Application

Research Category
Inflammation & Immunology

Research Sub Category
Immunoglobulins & Immunology

This Anti-phospho-IKK-epsilon (Ser172) Antibody is validated for use in WB for the detection of phospho-IKK-epsilon (Ser172).

Quality

Evaluated by Western Blot in CD3+CD28 treated and untreated Jurkat cell lysates.

Western Blot Analysis: 1:1,000 dilution of this antibody detected IKK-epsilon on 10 µg of CD3+CD28 treated and untreated Jurkat cell lysates.

Target description

~90 kDa observed

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide and 30% glycerol.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.

Analysis Note

Control
CD3+CD28 treated and untreated Jurkat cell lysates

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 1