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Key Documents

05-710

Sigma-Aldrich

Anti-NG2 Antibody, clone 132.38

clone 132.38, Upstate®, from mouse

Synonym(s):

Anti-CSPG4A, Anti-HMW-MAA, Anti-MCSP, Anti-MCSPG, Anti-MEL-CSPG, Anti-MSK16, Anti-NG2

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

132.38, monoclonal

species reactivity

rat, mouse

manufacturer/tradename

Upstate®

technique(s)

immunohistochemistry: suitable
western blot: suitable

isotype

IgG1

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

mouse ... Cspg4(121021)
rat ... Cspg4(81651)

Specificity

Recognizes NG2.

Immunogen

HEK293 cells expressing a truncated integral membrane form of NG2 consisting of amino acids 1592-2222

Application

Anti-NG2 Antibody, clone 132.38 detects level of NG2 & has been published & validated for use in IH & WB.
Cross-reactivity expected with mouse.
Research Category
Neuroscience
Research Sub Category
Neuronal & Glial Markers

Quality

routinely evaluated by immunoblot on whole rat brain preparation

Target description

~270-300 kDa

Physical form

Format: Purified
Protein A Purified immunoglobulin in 0.1M Tris-glycine, pH 7.4, 0.15M NaCl with 0.05% sodium azide as a preservative.
Protein G Purified

Storage and Stability

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
Brain tissue

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk_germany

WGK 1


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Sandra Stelzer et al.
BMC neuroscience, 11, 27-27 (2010-02-27)
Junctional adhesion molecule-A (JAM-A) is an adhesive protein expressed in various cell types. JAM-A localizes to the tight junctions between contacting endothelial and epithelial cells, where it contributes to cell-cell adhesion and to the control of paracellular permeability. So far
Yvonne M Ughrin et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 23(1), 175-186 (2003-01-07)
The NG2 chondroitin sulfate proteoglycan, an integral membrane proteoglycan, inhibits axon growth from cerebellar granule neurons and dorsal root ganglia (DRG) neurons in vitro. The extracellular domain of the NG2 core protein contains three subdomains: an N-terminal globular domain (domain
Dongying Chen et al.
Developmental biology, 407(2), 195-210 (2015-10-06)
Fibronectin (Fn1) is an evolutionarily conserved extracellular matrix glycoprotein essential for embryonic development. Global deletion of Fn1 leads to mid-gestation lethality from cardiovascular defects. However, severe morphogenetic defects that occur early in embryogenesis in these embryos precluded assigning a direct
Marijana Miljkovic-Licina et al.
Molecular cancer therapeutics, 11(12), 2588-2599 (2012-09-25)
Antiangiogenic drugs have been used as anticancer agents to target tumor endothelial cells or pericytes. Because of limited efficacy of the current monotherapies, there is a strong demand for the dual targeting of endothelial cells and pericytes. Here, we identify
Histone deacetylase expression in white matter oligodendrocytes after stroke.
Kassis, H; Chopp, M; Liu, XS; Shehadah, A; Roberts, C; Zhang, ZG
Neurochemistry International null

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